I recently popped out membranes from wafers 1064 and 1065 for our lab. I only started with about half a wafer, but these are the numbers from the extraction: Wafer ID # of In-Tact Extracted Membranes : High/Low Porosity #…
Category: NRG
FLAG Diffusion Experiment Progress Update
For context, see my previous post. Early on in the process of designing an experiment to confirm the usefulness of a CytoVu assembly for use as an assay, it was decided that the best solution for measuring the extremely low…
Posted in NRG
Zetapotential Measurements of pnc-Si, SiN, and Stripped SiN Membranes
My streaming potential device is now working consistently. It turns out that the problem was the concentrations of salt that I was using. The Debye Length (the distance over which charge-charge interactions are important in an electrolyte) is given as…
Posted in NRG
Endothelial Vacuoles Induced by Flow
In working on a long over due paper by Barrett on endothelial response to high permeability material … Key figs … Figure 2 Figure 4 Figure 5 And one post from Nakul. I came across this highly relevant paper Pedrigi(Overby)EER11
Posted in NRG
Review: Blood Purification 2013
In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two…
PDMS cell channel repeat
This is an update to my last post, where HUVECs mixed with collagen inside PDMS channels seemed to stay rounded up and not really do much of anything exciting. This time around I made a few adjustments: New collagen protocol…
Posted in NRG
Fluorescence Through Various Substrates – Statistical Analysis
In a previous post I had shown the relative fluorescence of various substrates as normalized to glass. 1um beads (either green or red) were placed onto each surface and imaged with constant EM Gain, exposure time, and intensity. Bar graphs were…
Posted in NRG
DAPI & Phalloidin Update – Thicker SiO2, SiN, Tissue Culture Plastic
In this previous post (also some in this post), I had displayed the difference between the 120nm SiO2 microporous membranes and four different iterations of Track-Etched insert membranes in reference to nuclear (DAPI, blue) and actin (Phalloidin, green) stain. I recently…
Posted in NRG
TEOS Membrane Co-Culture Devices (Version 2)
I’ve redesigned the co-culture devices described in an earlier post to allow for media isolation as well as to alleviate concerns regarding bubble trapping. The new design is pictured below: The large cube of silgard builds a cylindrical reservoir for…
Posted in NRG
Streaming Potential Revisited
After last week’s post detailing the problems I’ve been having with my streaming potential setup, Jirachai was kind enough to come by the U of R last friday to look at my device. We banged our heads into the wall…
Posted in NRG
