In vitro Clearance and Hemocompatibility Assessment of Ultrathin Nanoporous Silicon Membranes for Hemodialysis Applications Using Human Whole Blood Morteza Ahmadi Maud Gorbet John T.W. Yeow Department of Systems Design Engineering and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ont. , Canada Summary Two…
Category: NRG
PDMS cell channel repeat
This is an update to my last post, where HUVECs mixed with collagen inside PDMS channels seemed to stay rounded up and not really do much of anything exciting. This time around I made a few adjustments: New collagen protocol…
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Fluorescence Through Various Substrates – Statistical Analysis
In a previous post I had shown the relative fluorescence of various substrates as normalized to glass. 1um beads (either green or red) were placed onto each surface and imaged with constant EM Gain, exposure time, and intensity. Bar graphs were…
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DAPI & Phalloidin Update – Thicker SiO2, SiN, Tissue Culture Plastic
In this previous post (also some in this post), I had displayed the difference between the 120nm SiO2 microporous membranes and four different iterations of Track-Etched insert membranes in reference to nuclear (DAPI, blue) and actin (Phalloidin, green) stain. I recently…
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TEOS Membrane Co-Culture Devices (Version 2)
I’ve redesigned the co-culture devices described in an earlier post to allow for media isolation as well as to alleviate concerns regarding bubble trapping. The new design is pictured below: The large cube of silgard builds a cylindrical reservoir for…
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Streaming Potential Revisited
After last week’s post detailing the problems I’ve been having with my streaming potential setup, Jirachai was kind enough to come by the U of R last friday to look at my device. We banged our heads into the wall…
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Multi channel, dual chip dialysis device
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic
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Review of ACKD nanotechnology articles (Vol 20, issue 6, Nov 2013)
This is a cursory review of the nonotechnology articles appearing along with our paper in the Journal “Advances in Chronic Kidney Disease” Most of these are reviews and most deal with nanoparticles for either imaging or therapy. One does discuss…
Co-Culture on SiO2 3um Low Density Membranes
Scope: Human umbilical vein endothelial cells (HUVEC) and adipose derived stem cells (ADSC) were co-cultured on SiO2 low density 3um pore membranes. The ADSCs were initially seeded onto the membranes, at 6 hours the membranes were flipped over and placed…
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NSF Proposal: Tunable dielectrophoretic separation of nanoparticles with ultrathin microporous silicon membranes
Blanca, Tom, Aytug and I are working on a proposal to NSF that will be submitted Oct.29th to the Chem. & Bio. Separations Program of the CBET division. This is a joint 3-year project between Blanca’s and Tom’s labs. Basically,…
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