Back when I first joined the lab I did some experiments where I varied the pressure driving a gold filtration experiment. The critical plot was this:   It seems unlikely that the sieving coefficient could change so dramatically with applied…

Sealing Patch Clamp Chips Canadian Patch Clamp Chip   I have been thinking about Handy Geldbard’s group and what would enable them to study groups of synapses. Currently, I am in discussion with Jennetta Hammond and Pat Lu trying to…

I have been working with JP over the past month to attempt to form a thin, continuous film of PdAg on a pnc-Si membrane using e-beam evaporation.    The aim of this is to test these for selective hydrogen permeability at…

The group in the attached paper have found a way to form pnc-Si using PECVD.   They use a 300-15-300 nm OSO stack with a 850 C (50 C/s, 30 s) RTP to form ~13 nm pores.  SiMPore attempted this in the…

In my last post I reported findings that the bottom well of a CytoVu assembly is capable of adsorbing protein at about 0.03 ug/mL, or 0.75ng of protein. This is a fairly small value, but for the purposes of a…

Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films ABSTRACT: Despite considerable efforts toward fabricating ordered, water-permeable, mesoporous films from block copo- lymers, fine control over pore dimensions, structural character- istics, and mechanical behavior of graded structures…

Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells  and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used…

After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’…

In the wake of the apparent loss of all of the phosphatase in a sample that was placed into a CytoVu well, left for 24 hours, and then checked for phosphatase activity, it’s become clear that the adsorptive qualities of…

So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made…