In my last post I reported findings that the bottom well of a CytoVu assembly is capable of adsorbing protein at about 0.03 ug/mL, or 0.75ng of protein. This is a fairly small value, but for the purposes of a…
In my last post I reported findings that the bottom well of a CytoVu assembly is capable of adsorbing protein at about 0.03 ug/mL, or 0.75ng of protein. This is a fairly small value, but for the purposes of a…
Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films ABSTRACT: Despite considerable efforts toward fabricating ordered, water-permeable, mesoporous films from block copo- lymers, fine control over pore dimensions, structural character- istics, and mechanical behavior of graded structures…
Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used…
After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’…
In the wake of the apparent loss of all of the phosphatase in a sample that was placed into a CytoVu well, left for 24 hours, and then checked for phosphatase activity, it’s become clear that the adsorptive qualities of…
So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made…
EO tests were continued using 2 micron carboxylated PS Latex beads in a 0.2 mM K2HPO4 solution containing 0.1% tween. The solution had a pH of 7.54 and a conductivity of 67.0 uS/cm. The beads were negatively charged, and so…
This is a follow up to Josh’s post, “Continued Au Separations with SiN-NP wafer #’s 1029 and 1030”, and seems to explain why we initially got an S value greater than one during our gold separations. We conducted tests to…
As I mentioned in my previous post, there are a lot of problems associated with the technique for testing the ability of a CytoVu assembly to act as an assay that I had been trying to use before. Several possible…
I found some good descriptions of hemodialysis experiments using rats. They all use a method possibly first described by (Rockel et al., 1978). (Galons, Trouard, Gmitro, & Lien, 1996) at U of Arizona used nephrectomized male Spraque-Dawley rats (Holtzmann Co.,…