Category: NRG

Solutions to the Adsorption Problem

In my last post I reported findings that the bottom well of a CytoVu assembly is capable of adsorbing protein at about 0.03 ug/mL, or 0.75ng of protein. This is a fairly small value, but for the purposes of a

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Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films

Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films ABSTRACT: Despite considerable efforts toward fabricating ordered, water-permeable, mesoporous films from block copo- lymers, fine control over pore dimensions, structural character- istics, and mechanical behavior of graded structures

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ADSC differentiation and networking in various environments

Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells  and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used

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Semi-Quantitative Analysis of Optical Transparency 3µm Porous Membranes

After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’

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Returning to CytoVu Protein Adsorption

In the wake of the apparent loss of all of the phosphatase in a sample that was placed into a CytoVu well, left for 24 hours, and then checked for phosphatase activity, it’s become clear that the adsorptive qualities of

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Viability and growth of HUVECs on nanonitride membranes

So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made

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ElectroOsmosis and Particle Trapping in Microporous Membranes

EO tests were continued using 2 micron carboxylated PS Latex beads in a 0.2 mM K2HPO4 solution containing 0.1% tween. The solution had a pH of 7.54 and a conductivity of 67.0 uS/cm. The beads were negatively charged, and so

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Absorbance Problems with Colored Centrifuge Tubes

This is a follow up to Josh’s post, “Continued Au Separations with SiN-NP wafer #’s 1029 and 1030”, and seems to explain why we initially got an S value greater than one during our gold separations. We conducted tests to

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FLAG Separation Experiments with an Enzymatic Activity Assay

As I mentioned in my previous post, there are a lot of problems associated with the technique for testing the ability of a CytoVu assembly to act as an assay that I had been trying to use before. Several possible

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Hemodialysis in the rat animal model

I found some good descriptions of hemodialysis experiments using rats. They all use a method possibly first described by (Rockel et al., 1978). (Galons, Trouard, Gmitro, & Lien, 1996) at U of Arizona used nephrectomized male Spraque-Dawley rats (Holtzmann Co.,

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