Category: NRG

Fluorescence Focal Test – 300nm Microporous Oxide Membranes Do Not Wrinkle or Sag After Autoclaving

Method: Oxide membranes in some conditions have been shown to wrinkle over time.  To study this, three different varieties of SiO2 membranes were observed (1000C nonporous 120nm SiO2, 150W deposited microporous low-density 300nm SiO2, and 100W deposited microporous low-density300nm  SiO2).…

Posted in NRG

PEG Mono-layer Detection on Crosslinked Oxide Chips

Below are the results of my recent crosslinking efforts. PEG with 1.5% crosslinker was exposed to UV light at varying times through a bar  printer/transparency mask measuring 100/200. In theory this corresponds to 100 micron PEG bars and 200 micron…

Posted in NRG

Co-Culture of ADSCs and HUVECs

Scope: Adipose derived stem cells were seeded onto  Corning 3um tracketch membranes, human endothelial derived stem cells were seeded onto the membranes approximately five hours later. Numerous control wells were also seeded with ADSCs both on membranes and on the…

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TEOS Membrane Co-Culture Devices

Over the past week Andrea and I designed a new co-culture support structure for use with SiMPore’s 5.5 mm square membranes. The devices are built for use in standard 24-well plates and should prove useful to anyone wanting to do…

Posted in NRG

Magnesium Fluoride Nanoporous Membranes

Wet Etching of Optical Thin Films   As a result of Christian’s trip and Kevin’s discussions with me, I have been wondering if we could make nanoporous MgF2 membranes for Raman spectroscopy. Part of the appeal in using our Si-based…

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Co-Culture of ADSCs/HUVECs on 0.4um Translucent Membranes

Scope: ADSCs were cultured on the bottom of the Corning 0.4um translucent membranes in DMEM+ 10% FBS+ 1% Pen-Strep. After 6 hours, the membranes were flipped over and placed into a 24 well plate. Of the four membranes with ADSCs…

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SSI RTP software updated to version 3.8

I updated the software for the SSI.  The software seems to be working properly.  There are some differences, most of which I haven’t fully explored.  Notably, we can open and view log files easily with 3.8. It seems that we…

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EOF&DEP simulations for 300 nm membranes

We have considered SiN membranes in PDMS channels as EOF pumps and as constrictions for insulator-based dielectrophoresis (iDEP). For EOF, thicker membranes may yield greater flow rates. For iDEP, a smaller membrane porosity, meaning fewer, smaller holes on a given…

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Viability and growth of HUVECs on nanonitride membranes – Part 2

This is in continuation to the studies that I published here. I used two types of membrane surfaces: nano nitride trenches (the back side of membranes) with pure nitride on the surface, and zero-second-etch nano nitride with a completely intact…

Posted in NRG

Boron doped c-Si substrate to create silicon lattice under membrane

Hopefully many more details to follow, but JP, Josh M. and I doped some Si substrates with boron using a spin-on-glass.  It seems well known that heavily boron doped Si is more resistant to EDP etch than intrinsic or n-type…

Posted in NRG
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