We have seen before that silanization slows down discoloration and hence prevents membrane degradation. In Sepcon, the sample should last over a week. Further, it was necessary to check whether silanized surface supports normal cell growth. Therefore, comparison of cell…
Tag: cell culture
Silanization and Membrane Chemical Stability
Due to our desire to improve membrane stability in cell culture, different methods of treatment have been mentioned to try, including carbonization and silanization. W606 was treated with UV/ozone at 150C for 20 minutes, followed by silanization performed by some…
Membrane Stability under various RTP Treatments: Retry
Previously W514 was tested with different RTP conditions (varying time at 800C, all in Ar); the results were difficult to interpret as the cells were confluent (in some cases it was hard to see membrane breakage). We therefore decided to…
HUVEC spreading on pncSi and glass
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs. HUVEC spreading on pncSi HUVEC spreading on glass
Chemical Stability of 30nm Samples with RTP Treatment
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant…
Rhodamine diffusion profiles for non-rtp/rtp pncSi
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
Fibroblasts spreading on glass
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on…
Cell Spreading on pncSi and glass
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time…
Membrane stability in cell culture: various treatment conditions
Four different post-production treatments with the objective of improving membrane stability in culture were run last week on W615, a pinholed wafer laden with the circular features we have been seeing recently. I ran four different conditions for cell culture:…
Rhodamine 6G diffusion through RTP treated and non-treated pncSi (w/o pinholes)
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no…
