W613, the wafer with 30 nm thick pnc-Si was tested for chemical stability. This wafer was shown to be very weak mechanically, with burst pressures less than 1 PSI. I tested this wafer for chemical stability with cells (fibroblasts, P26)…
Tag: cell culture
RTP Time Variation for Cell Culture
In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at…
Membrane Stability in Cell Culture
Last week I ran cell culture growth experiments on RTPed (800C 5 minutes in Ar) samples from W567 (single slit 100um x 1000um). For this study I tracked discoloration w/cells, membrane stability w/cells, and the bead assay w/cells to see…
Fibroblast spreading on the membrane
Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours. I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture…
W567 Burst Pressure and Air Permeability
I recently received wafer 567 from Dave for cell culture work. It has many intact membranes but is riddled with pinholes, having only 3 pinhole-free samples. I decided to test them for air permeability and burst pressure, shown below. I…
HUVEC adhesion update
Couple of HUVEC adhesion trials were carried out and the results were found satisfactory as seen in the charts below.
HUVEC growth kinetics with serum free media as control
HUVEC growth was monitored for 4 days on different substrates (glass, plastic and pncSi), this time in media (EGM) with and without serum. Growth in media w/o serum was observed to be much slower than the growth in media with…
HUVEC Adhesion with controls (repeat)
HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as…
HUVEC Adhesion with Controls
HUVEC adhesion was measured on glass, tissue culture plastic, and pnc-Si, with poly-lysine coated plastic and teflon coverslips as positive and negative controls. Adhesion on pnc-Si was low, this could be due to cells being lost through broken slits or…
Fibroblast adhesion study using controls
Fibroblast adhesion experiment was done using poly-lysine coated plastic and teflon coverslips as positive and negative controls repectively. Poly-lysine is a polypeptide that gives the cells a sticky surface to adhere to. Teflon coverslips are used as negative control because…
