Four different post-production treatments with the objective of improving membrane stability in culture were run last week on W615, a pinholed wafer laden with the circular features we have been seeing recently. I ran four different conditions for cell culture:…
Tag: karl
Chemical Stability of W613
W613, the wafer with 30 nm thick pnc-Si was tested for chemical stability. This wafer was shown to be very weak mechanically, with burst pressures less than 1 PSI. I tested this wafer for chemical stability with cells (fibroblasts, P26)…
W613: Mechanical Testing
W613, the wafer with 30 nm thick pnc-Si that is oddly etched to form “H” shaped membranes, was tested for burst pressure and attempted to measure air permeability. When doing air permeability all samples broke, meaning are very weak. This…
W620: Mechanical Testing
The production crew recently delivered W620 to the lab, which was created to mimic W504 (and W612). W620 shows high burst pressure and has air permeability comparable to W504/612.
Desalting with Intact Membranes: Continued
As mentioned previously intact membranes show a flux comparable to membranes with pinholes during desalting experiments. To confirm this a third intact membrane test and two pinholed tests were run under the exact same conditions. Previous pinholed samples had different…
Change in Pore Image Processor
Yesterday I found a bug in the pore image processor. The “i” hotkey is supposed to save the current image, but it wasn’t working. I contacted Mike Bindschadler and he fixed it. The updated version can be found here. The…
Attempt at UV/Ozone to Clean Membranes of Organic Impurity
I tried to clean samples from W615 using UV/ozone to cleanse the membranes of the organic impurities we have been seeing lately. I followed the UV/ozone protocol, with a stage temp of 25C and treatment time of 5 minutes. No…
W612 Characterization
I tested W612 for air permeability and burst pressure, shown below. It should be noted that the air permeability and burst pressure values are comparable to W504; looks like a good reproduction! I also tested a sample with the hexane…
RTP Time Variation for Cell Culture
In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at…
Desalting: Intact SepCons
Introduction One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Ideal membranes have uniform pore distributions, high mechanical strength, tunable, sharp pore cutoffs, and high porosity. In…
