We’ve long speculated that adding fluid flow over our tissue models would reduce the concentration of pro-inflammatory factors secreted in response to inflammatory challenges, dampening the overall inflammatory state compared to static culture. We now have evidence that this is…
Author: Isabelle Linares
Developing a Microfluidic Human Tendon-on-a-Chip to Investigate the Role of the Vasculature in Tendon Injury – MPS World Summit 2024 Poster
Isabelle Linares_MPS World Summit 2024_Poster
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The Journey to a Manufactured Flow Insert + Protocol for Use
Introduction The modular μSiM is based on a 96-well plate format with a 100 μl open well. While these devices have proven extremely useful for various barrier models including the blood-brain barrier1 and the tendon-vascular interface2, there is a need…
Manufacturable Single-Slot Flow Insert Feasibility Testing
Introduction As discussed in a previous post, we are working to adapt a PDMS flow insert as a manufacturable component. While our priority was maintaining the original flow channel geometry as Mansouri et al. designed, we slightly changed the channel…
Manufacturable Single-Slot Flow Insert Update
Introduction A recent collaborative publication from RIT developed a modular µSiM to address the lack of physiological fluid flow in conventional open-well devices (Figure 1). The magnetic latching assembly of this design allows for quick configuration into a fluidic setup.…
Fabricating Thin Polymer Coatings to Study S. Aureus Durotaxis – PUA Optimization
Introduction The work in this post explores the hypothesis that durotaxis affects the invasion of S. aureus into the osteocyte lacuna-canicular network (OLCN). To address this hypothesis, we modified the µSiM to enable the independent tuning of pore stiffness, ranging…
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Fabricating Thin Polymer Coatings to Study S. Aureus Durotaxis – Stiffness Measurements
Introduction The invasion of Staphlyococcus aureus into the cracks of cortical bone leads to osteomyelitis, a devastating disease often resolved by amputation [1]. The μSiM platform has been used to study the genetic mechanisms of S. aureus invasion. The membrane…
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