Karl and I have been working to measure separations using SEPCONs with particles ranging in size from 20 nm to 1 µm. We started out naively enough by slightly diluting some flourospheres we found in the refrigerator, putting them in the…
Tag: separation
A quick graph showing that a higher active area/inactive area ratio sees less pore blockage
These volume measures were conducted at 5.6 +/-0.2 PSI using the variable area sepcons with either a half slot, one slot, three slots, or the normal five slots. Note that the pressure was normalized via a pressure regulator, and…
Posted in NRG Tagged with: BSA, pressure, pressure setup, separation, streamline convergence?, TECAN, variable area
Long Term Urea Clearance in Serum
I made 50 mL of 30% fetal bovine serum (FBS), 70% PBS, 0.5 mM urea. Pumped with syringe pump at 5.6 µL/min through an untreated and EA treated single slot dialysis chips in a stirred beaker of PBS (two separate…
More details on INT cartridge's "desalting" column
First things first, the desalting column is not intended to desalt. Apparently that’s just an old name that has carried over. The current purpose is to separate viruses from large debris after sonication. The sample in a buffer solution will…
New Cartridge from INT
INT received the first samples of their new cartridge design. The following slides were given to us by INT to provide some detail. The shorter cylinder on the left that is called the “glass bead pod” needs on filter fixed…
Pressurized Gold Separations
Motiviation and Background: Nakul and I have been performing a lot of DNA filtration and attempted DNA shearing experiments. We found that large DNA fragments (>1000 and >2000 bp) pass through ~30-40 nm max pore size membranes when pressurized to…
Gold Trials
Most recently I’ve been working with colloidal gold nanoparticles (referred to as AuNP from here on). We chose to use AuNP for separations because it is an ideal hard sphere particle, unlike a floppy protein. We thought we could achieve…
