We have had success cross-linking PEG to TEOS membranes pretreated with TPM to aid in adhesion. This was done first on nonporous membranes, and then porous. We used 50% PEG at 1.5% cross-linker, which cross-linked at 8000ms. For comparison, 100%…
Category: NRG
PLA Plastic Acidifies DMEM + 10% FBS
I have been looking into PLA (Poly Lactic Acid) plastic for use in our microfluidics. PLA is a commonly used material in 3-d printers, so it would be great if we could print out our microchannels for cell-culture devices.…
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SiN-NP with varied RIE times
We used 4″ wafers with 40 nm pnc-Si on 50 nm RV SiN (RTP 1050 C, 100 C/s, 60 s). JP ran a series of RIE times from 10 to 50 s with 10 s intervals. (We had initially planned on…
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Scanning ion conductance microscopy of cells on TEM grids (pnc-Si, not annealed)
Bend.3 + Geltrex Tube Formation Studies
The following diagram lays out each separate setup that was tested using different combinations of Geltrex and substrates. Each trial has a different reasoning behind why it was done. The purpose of these trials was to investigate the effects of…
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In-situ heating experiments on the OSO and NSN samples
I have been working on the in-situ heating experiments on the free-standing membranes in the past few weeks. After quit a few times attempts I finally have some good videos made from the TEM images that were taken during the…
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Microporous Silicon Dioxide (TEOS) vs. Nitride Imaging
After a false start with non-microporous TEOS chips, I quickly did an image comparison with truly porous chips using polystyrene beads. The TEOS and Silicon Nitride chips were both fabricated in the single-well CytoVu format (single 2 mm window, 5.4mm…
Long Term Urea: Evaporation measurement and Pressure Calculations.
The initial measurements with multiple tests tubes containing either Serum or PBS failed to demonstrate evaporation and in fact the weights of the collection tubes increased after sitting in the refrigerator overnight. Perhaps the tape collected water? At any rate…
Filtration of DNA and Bugs @ INT
Two experiments were performed to explore application of SiMPore filters within INT’s microfluidic cartridges to allow the capability for pathogen type specificity in nucleic acid purification. 1) The first experiment tested the efficiency at which nucleic acids could pass through…
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Membrane Permeability Testing
Summary of Results Membrane Pore Diameter Thickness Porosity Permeability (cc/cm^2*min*bar) SiN 3.2-4.0 um 0.12 um 22.1%-35.3% 30000 PCTE 2.4-3.0 um 9 um 11.9%-16.1% 1652 SiN Experimental Design Permeability of the 3 um pore Si Nitride membranes was assessed using the…
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