Discussion: The BSA may have not been fresh enough to give good data, the difference between background and signal are pretty low. The Rhodamine IgG gave better signals but the PEG4 treated SiN is between the untreated SiN and bare…
Tag: BSA
Permeability Graphs for BSA Separations with Variable-Active-Area Sepcons Show Flow is Independent of Fractional Active Area
What I had in my two previous posts described as permeability graphs were in fact total volume passed/ total time passed graphs: What I thought I saw was that cake layer formation was dependent on the filter geometry. There is…
"Phantom" Curves on the TECAN Mess with Reproducibility and Accuracy
At some point, when things settle down, I’ll create a page dedicated to the TECAN machine and document all of the troubleshooting and tricks I’ve learned so far. Before that happens I have another mystery to solve. Take a look…
A quick graph showing that a higher active area/inactive area ratio sees less pore blockage
These volume measures were conducted at 5.6 +/-0.2 PSI using the variable area sepcons with either a half slot, one slot, three slots, or the normal five slots. Note that the pressure was normalized via a pressure regulator, and…
Posted in NRG Tagged with: BSA, pressure, pressure setup, separation, streamline convergence?, TECAN, variable area
f-BSA adsorption on Functionalized pnc-Si (Shestopalov Lab)
Katia in Dr. Shestopalov’s Lab functionalized some pnc-Si windowless chips. The process she follows is shown in Figure 1. She provided chips at various stages of the process. “Grignard”, “Diazirine”, and “PEG”. After functionalization, the chips were wetted with…
BSA Retention in Single Channel Dialyzer
Regarding the single channel dialyzer chip with 20-nm thick nano-porous silicon membrane: 10 mm long, 300 µm deep, 500 µm wide channels. Now that we’ve shown that a measurable quantity of urea is filtered out of DI water in one…
