A group published a paper in Lab on a Chip (2012) using track-etched membranes for electro-osmosis. There are a lot of eerily familiar terms and concepts in the paper. The title even sounds familiar: Low-voltage electroosmotic pumps fabricated from track-etched…
Category: NRG
Shadow Mask ITO Deposition Characterization
Josh has been kind enough to fabricate a shadow mask for me to create interdigitated electrodes without the hassle of photolithography and etching. In order to be able to use this method, I want to make sure the produced electrodes…
Posted in NRG
TRITC tagging of Fetal Bovine Serum, TECAN v ImageJ measurements
pnc-Si chips prewetted with PBS then incubated with fetal bovine serum (unknown molarity) for 2 hours under refrigerated conditions. Thoroughly rinsed with reaction buffer (perfused 5 times). TRITC (2 mg/mL) added to dry chips and incubated at room temperature for…
The Concentration of the Barcikowski Peptide is at the Lower Bound of Our Detection Limit
Recall that we’ve been having a great deal of trouble following the free ligand during our separations of the Bioconjugated Barcikowski particles, and that this trouble also means we can’t see the ligand after centrifuging the pure sample. In the…
Posted in NRG
Five-layer structure (2)
In order to verify the validity of the result of the five-layer structure I did last time, I deposited another wafer with NOSON stack, which has the identical thickness as that of the last time. The annealing condition is also…
Posted in NRG
200nm thick Si film from the NSN stack
During my visit last, I deposited a couple of wafers and one of them is to test a even thicker Si film from the NSN stack. The thickness for the Si film is 200nm and the nitride layers are still…
Posted in NRG
Comparitive Functionalization of pnc-Si measured with f-BSA fluorescence intensity
The last of the larger windowless pnc-Si chips were sent to both Adarza and Dr. Shestopalov’s lab for functionalization. Adarza returned chips with PEG-1 and PEG-2 (different rounds of processing) with both 2 hours and 16 hour treatments. They also…
Shear-free chemotaxis: potential room for improvements
One major problem that we faced with our current shear-free chemotaxis system is the lack of high image quality. Since the silicon-nitride (SiN )grid is 100 um away from the coverslip surface on which the cells migrate, the out-of-focused SiN…
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Shear-free chemotaxis: nanoparticle tracking showed significant flow reduction conferred by the membrane
To assess how shear-free our [ “shear-free” chemotaxis system] is, we tracked the motion of nanoparticles in the shear-free compartment as we input a known flow rate in the flow compartment. The nanoparticles we use are the 210 nm dragon green…
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Shear-free chemotaxis: linear gradient maintained after passing through the membrane, assessed via TIRF
Hi all, We have already showed in our earlier [ post ] that our shear-free chemotaxis system can indeed elicit chemotaxis, suggesting that we have successfully translated a gradient through the membrane to the shear-free side of the system where the cells…
Posted in NRG
