This is a continuation of earlier work showing 30-100-30 NSN x-sections where the Si layer thickness increases from ~92 nm before RTP to ~130 nm after RTP. Below are SEMs showing a ~50 nm thick a-Si film becoming a ~62 nm…
Category: NRG
SEMs of burst 30-50-30 NSN SEPCON
Below are some SEM images of a burst 30-50-30 NSN membrane in a SEPCON pattern. Many similar chips failed at lower pressures, but this one survived to ~5-7 psi. (didn’t keep close track of the chips after they were burst,…
Counter Flow Dialysis Device Design
Upper and lower gaskets used to make a counter flow device. The danger is that the enclosing pdms can easily flex into the membranes on the flat side of the chip. A hard plastic cover can be used to prevent…
f-BSA Adsorption on Adarza functionalized pnc-Si chips
Chips of pnc-Si were funtionalized at Adarza. They returned four groups of chips. Ethanolamine, Amino PEG-1, Inactivated sLinker, and Amino PEG-2. These were all incubated with PBS and 5-mg/mL f-BSA (2:3) in a humid refrigerated dish. The samples were rinsed…
Pore forming conditions
In the previous posts, I have shown that nanopores can be formed in silicon film sandwiched by two silicon nitride films. This discovery shows silicon nitride as an alternative barrier layer in the stack geometry. In this post, I’ll discuss…
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BBB Device V2.6 Flow and TEER Test
Following my fabrication process from my previous post, here are the results from my flow test of BBB device v2.6: The difference in the quality of the electrodes is apparent from these images. The microscope slide electrode was difficult to…
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Burst Pressure, Permeance of NSN pnc-Si SEPCONS with large pores
Karl, Charles and I measured the burst pressure and permeance of some 30-50-30 and 30-100-30 NSN. Burst Pressure: The SEPCONs readily burst at pressures under 1 psi when the flow was directed from the backside of the chip through the…
micro-porous lift-off TEOS
For this experiment, 3um diameter pores were created using SimPore’s mask. Reactive Ion Etching was used to form the pores in the SiN. Both 25nm and 150nm TEOS oxides were used. An SU8 Structure of 10um lines with 100um square…
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Filtration of Bioconjugated Samples Retains Gold, but We Can't Follow the Ligand
On Friday(which was a great day for science). I ran a series of 5 passes of the bioconjugated gold nanoparticles through 30nm cutoff Gen 3 Sepcons. If you’ll remember, the Barcikowski lab wants the free ligand separated out from the…
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Inventory of Nanoparticles
Nanoparticles Inventory – taken by Karl on 11/2/12. All these particles can be found in the refrigerator. Manufacturer size type amount (approx) expiration date fluorescence (if applicable) BBI 5nm gold colloid 3mL Jan-10 BBI 5nm gold colloid 6mL Aug-11 BBI…
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