Yesterday I started a test to try and reproduce the results Henry was able to obtain with a closed flow system last week. Using four samples (W619) I ran the UV Ozone bonding process and allowed them to sit over…
Category: NRG
W612 Characterization
I tested W612 for air permeability and burst pressure, shown below. It should be noted that the air permeability and burst pressure values are comparable to W504; looks like a good reproduction! I also tested a sample with the hexane…
RTP Time Variation for Cell Culture
In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at…
Closed Flow System Update
Just an update on the closed flow systems I put together yesterday using vacuum grease. Even with the vacuum grease at the point of entrance of the capillary tube to the PDMS-pncSi system the PDMS was able to get into…
Human Albumin/Cytochrome C diffusion cell
I ran another diffusion cell last week using Human Albumin and Cytochrome C. I increased the volume of PBS because the last time I ran the gel for this mixture, the band for cytochrome c was very prominent. Out of…
Posted in NRG
AJA membrane
While Chris and I were in Boston we deposited our membrane stack on a patterned wafer. It was annealed at 1000 C and then etched. Because we didn’t have an accurate deposition rate for Silicon, we overestimated and put down…
Posted in NRG
Wafer 612 and 615
Wafer 612 was made to mimic wafer 504: 850 C RTP, cool down, 1000 C RTP. Wafer 615 was annealed in two steps: first to 400 C and then to 1000 C (without inbetween cool down). Both wafers showed a…
Posted in NRG
Desalting: Intact SepCons
Introduction One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Ideal membranes have uniform pore distributions, high mechanical strength, tunable, sharp pore cutoffs, and high porosity. In…
W612 Pinhole Counts: Interesting Features
I am in the process of inspecting each membrane in the newly created wafer 612. First of all, some samples (~10% counted so far) have tears on the membrane edges, shown below. Images were taken at 20x microscope magnification (reflection…
Closed Flow System Update
Just an update on the PDMS-pncSi closed flow systems progress… The issues we were having early last week with bonding seem to have been solved. We used to run extra ozone flow for ten minutes after the initial 5minutes of…
