HUVEC growth was monitored for 4 days on different substrates (glass, plastic and pncSi), this time in media (EGM) with and without serum. Growth in media w/o serum was observed to be much slower than the growth in media with…
Category: NRG
PDMS-Membrane Bonding Update
This is just an update on the progress being made towards creating a closed flow cell by bonding PDMS to our membrane chips. For those who were not at the meeting on Tuesday we have successfully bonded PDMS to both…
DI water buffer or thiol passivation?
I hope to bring this question to our group meeting. As we discussed before, we know that Cl- can be absorbed at the gold surface to screen the applied positive charge. We are using R6G as the filtration species, which…
Posted in NRG
Desalting: Broken and Pinholed Membranes
Desalting of 4M NaCl in deionized water was performed using our membranes in the SepCon format. I ran the SepCons in the same as way as the previous study, using a low stir rate and floating the SepCons. Once again,…
Membrane anneal study
Last week, we reported that it was possible to anneal membranes after they had been etched. Below is a summary of results from membranes that were annealed over a range of temps and under different conditions. w600 (20 nm oxide)…
Posted in NRG
new chamber with brief description
As per Chris request, here is a photo with a brief description. Sorry the image is still not good… the surface of the plastic reflects lights and it is transparent, plus I am using a poor camera. But I hope…
Posted in NRG
new version of the diffusion chamber
Since Paul sent an email saying he will be on leave from this week, I spent some time in the hopeman metal workshop with Paul working on our second diffusion chamber before he leaves. We actually failed four substrates before…
Posted in NRG
diffusion observed using the prototype chamber with background noise
Last week Jess and I did some diffusion tests using the prototype chamber. We observed there was some fluorescence background noise from the plastic substrate. So we decided to make the buffer well (the hole on the substrate) all the…
Posted in NRG
Success with PDMS-Glass Bonding
Yesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room…
W509X Burst Pressure and Air Permeability
W509X, with single 100um by 1000um slits, was tested for air permeability and burst pressure. Prior to testing, I inspected all membranes for pinholes.; every sample had pinholes on the membrane perimeter, as shown below. The first two images are…
