Henry and I just got back from our meeting with Dean over at RIT to talk with him about the production process for making our proposed flow dialysis system. While we were there Dean described in detail the entire production…
Category: NRG
Diffusion Stir Cell Update
Mike and I tested the movement of Rhodamine between the two cell channels to prove that the Rhodamine particles do diffuse through the membrane. After inspection in the microscope we saw that the fluorescence from the Rhodamine does transfer from…
Adhesion Testing: HUVEC and Fibroblasts
Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities…
HUVEC growth curve
HUVEC (P5) growth was monitored over 3 days on different substrates (glass coverslips, tissue culture treated & untreated plastic and RTPed pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate…
Electron Beam Evaporator Protocol
Electron Beam Evaporator Protocol (V_1.0) – Lingyun Here is the first version protocol of the electron beam evaporator at RIT clean room. There is not too much to put down here – the best way to learn this is to…
Fibroblast Growth Take 3
A third fibroblast (L1 3T3, P22) growth test was conducted using glass, untreated and tissue culture treated plastic, and RTPed pnc-Si (W509) as substrates. Cells were grown in DMEM +10% FBS changed every 48 hours. R values are for the…
New metal coated wafer#502
Wafer#502 was coated with 10nm_Ti & 15nm_Au on Friday. I will pick out pinhole free membranes later today. I will also try to get TEM images at Karen’s lab (medical center). // The RIT clean room is closed this week.
Adsorption Quantification I
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done.…
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BSA Adsorption Studies 2
In our previous adsorption study, all of the surfaces seemed to adsorb a similar amount of protein. We thought this might be due to ineffective washing. In this study I tried a better washing technique: Incubate polymer membranes, pnc-Si chips,…
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Fluorescence Adsorption Assays
Glass chips and pnc-Si membranes were incubated in 1mg/mL FITC labeled BSA for 30min. They were vigorously washed in a large beaker of PBS. Chips were placed on a slide and imaged with fluorescence scope. For this study, it seems…
Posted in NRG
