Just wanted to let everyone know that the fused silica capillary tubing and adapter needed to connect them to the pump tubing have been ordered and should be in hopefully by Friday (Monday at the latest).
Category: NRG
New diffusion chamber prototype
Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is…
Posted in NRG
pH and Salt test on pncSi
We have been wondering about the reason for discoloration for a while. This could be due to the pH of the solution or the concentration of the salts in the solution. To narrow down our vision on this, first we…
HUVEC and Fibroblast Growth Summary
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are…
Dialysis System Update
Henry and I just got back from our meeting with Dean over at RIT to talk with him about the production process for making our proposed flow dialysis system. While we were there Dean described in detail the entire production…
Posted in NRG
Diffusion Stir Cell Update
Mike and I tested the movement of Rhodamine between the two cell channels to prove that the Rhodamine particles do diffuse through the membrane. After inspection in the microscope we saw that the fluorescence from the Rhodamine does transfer from…
Adhesion Testing: HUVEC and Fibroblasts
Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities…
HUVEC growth curve
HUVEC (P5) growth was monitored over 3 days on different substrates (glass coverslips, tissue culture treated & untreated plastic and RTPed pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate…
Electron Beam Evaporator Protocol
Electron Beam Evaporator Protocol (V_1.0) – Lingyun Here is the first version protocol of the electron beam evaporator at RIT clean room. There is not too much to put down here – the best way to learn this is to…
Fibroblast Growth Take 3
A third fibroblast (L1 3T3, P22) growth test was conducted using glass, untreated and tissue culture treated plastic, and RTPed pnc-Si (W509) as substrates. Cells were grown in DMEM +10% FBS changed every 48 hours. R values are for the…
