Category: NRG

Optimization of our isolation technique using Beads

Hello Everyone, I have been working on 100 and 50 nm beads since ACNMM conference to optimize our isolation technique for exosomes and verify that our approach works. Technically, based on the discussion that we had before about Peclet Number,…

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Latest 2-chip Small Animal HD results

While the latest small animal studies was meant to be a test of the new 1-chip bonded HD devices, device failure resulted in the use of the older 2-chip 10-channel HD devices as used on previous experiments. Here is all…

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PEG-NPN for Reduced Protein Binding

Hopefully in the next week we will be submitting the following manuscript … X. Li, D. Johnson, W. Ma, H. Chung, J. Getpreecharsawas, J.L. McGrath, A.A. Shestopalov, Modification of Nanoporous Silicon Nitride with Stable and Functional Organic Monolayers, ACS Chemistry…

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Microporous MgF2 summary

I’ve been gathering data for a paper using my microporous MgF2 nanomembranes so here are some sample figures for such a paper. Herein, we are trying to create a porous, Raman-compatible substrate for use in cell culture. Researchers have used…

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DNA translocations through ALD-coated NPN

First off, a quick update on the state of the lab. The day finally came, we had to pack up and put everything in storage, so unless there is a very pressing need, I’m done data collection for the time…

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Review of nano-fabrication

Hello everyone, for my first ever post I would like to share with you a collection of ideas that has turned into a review I’ll be working on. Here you will find some selected publications that report interesting fabrication processes…

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TAT ELISA study for substrate hemocompatibility

This post is an update of the TAT ELISA study. Details of which can be found in the prior post by Keisha and I: https://trace-bmps.org/blog/data/2016/02/18/hemocompatibility-studies This is the most up-to-date data: The error bar represents the standard error of mean. n…

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C3a ELISA study for substrate hemocompatibility

Purpose: Study the hemocompatibility of different substrates using the concentration of C3a as the indicator. Background: C3a is a protein produced during immune response. Directly from Wikipedia: The classical pathway of complement activation is initiated when the C1 complex, made…

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YAP/TAZ Passage One Staining

After reading and discussing a paper in journal club that talked about cells having a “mechanical memory” (found here “Mechanical memory and dosing influence stem cell fate“), we decided to replicate our previous YAP/TAZ experiments with passage one HUVECs direct from…

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Microporous (0.4 um) MgF2 has roughly the same material purity as nanoporous MgF2

I was concerned that even though my calculations for etching the microporous MgF2 material show that the silicon nitride was being removed completely, not all of the silicon nitride was indeed being removed. Hypothetically, the presence of additional remnants of silicon…

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