Category: NRG

DNA translocations through ALD-coated NPN

First off, a quick update on the state of the lab. The day finally came, we had to pack up and put everything in storage, so unless there is a very pressing need, I’m done data collection for the time

Posted in NRG

Review of nano-fabrication

Hello everyone, for my first ever post I would like to share with you a collection of ideas that has turned into a review I’ll be working on. Here you will find some selected publications that report interesting fabrication processes

Posted in NRG

TAT ELISA study for substrate hemocompatibility

This post is an update of the TAT ELISA study. Details of which can be found in the prior post by Keisha and I: https://trace-bmps.org/blog/data/2016/02/18/hemocompatibility-studies This is the most up-to-date data: The error bar represents the standard error of mean. n

Posted in NRG

C3a ELISA study for substrate hemocompatibility

Purpose: Study the hemocompatibility of different substrates using the concentration of C3a as the indicator. Background: C3a is a protein produced during immune response. Directly from Wikipedia: The classical pathway of complement activation is initiated when the C1 complex, made

Posted in NRG

YAP/TAZ Passage One Staining

After reading and discussing a paper in journal club that talked about cells having a “mechanical memory” (found here “Mechanical memory and dosing influence stem cell fate“), we decided to replicate our previous YAP/TAZ experiments with passage one HUVECs direct from

Posted in NRG

Microporous (0.4 um) MgF2 has roughly the same material purity as nanoporous MgF2

I was concerned that even though my calculations for etching the microporous MgF2 material show that the silicon nitride was being removed completely, not all of the silicon nitride was indeed being removed. Hypothetically, the presence of additional remnants of silicon

Posted in NRG

Dielectrophoresis Induced by a Nanoporous Membrane

It is expecting that the high electric field gradient at the nanopore entrance could amplify the dielectrophoretic force so that the nanoparticles transported toward the membrane could be manipulated based on their polarizabilities.  To prove the concept, the electric field

Posted in NRG

Ensuring cell adhesion on microporous substrates under shear flow

Just to give a brief background, I have been working with endothelial cells to make a microvessel mimetic device, and one of the important requirements for endothelial cell culture is to be able to grow the cells under physiological levels

Posted in NRG

YAP/TAZ Staining on Membranes and Nonporous SiO2

As we’re uncovering more bits and pieces of data, we kept being lead to the idea that our membranes may be made out of SiO2 and have a modulus in the gigapascal range, but cells seem to perceive them as soft

Posted in NRG, Protocols

Size separation of DNA with NPN pre-filters

Since my last update I have been trying to achieve reliable size-separation of DNA by length using dwell time with the NPN pre-filter. Originally I had hoped to achieve 250-bp resolution near the optimal length for the NPN filter devices

Posted in NRG
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