Paper for Friday’s Journal Club … Watch this space for a summary and analysis. Pensabene(Hofmeister)ACSAMI16 Synopsis 2um(?) pores in 100 nm thick PLLA. Very, very low porosity. Fabricated with a PVA microneedle mold produced with femto second laser. Microfluidic device…
Category: NRG
COMSOL Model Files Download Page
This post is not an update on my research. I am creating this page to house download links to the COMSOL Multiphysics models I’ve constructed in the course of my work here in the McGrath Lab. This page is linked…
Posted in NRG
Solid- State Nanopores Integrated with Nanoporous Membranes for Enhanced Single-Molecule Counting of Low-Abundance Biomarkers
We are putting the final touches on a submission to NIH based on our work with Vincent Tabard-Cossa in Ottawa. I want to provide everyone with a small overview. The basic premise is that solid nanopores (ssNPs) are the right…
Posted in NRG
Investigating the endothelial basement membrane formation on SiO2 membranes
In our prior work we showed that endothelial cells form far fewer focal adhesions on microporous SiO2 membranes compared to non-porous SiO2 and tissue culture plastic (see prior post by Ana). We performed this analysis at 24 hours, which seems…
Posted in NRG
Journal Club: Residual matrix from different separation techniques impacts exosome biological activity
Residual matrix from different separation techniques impacts exosome biological activity (PDF) Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta & Paolo Bergese. Scientific Reports 6, Article number: 23550 (2016) doi:10.1038/srep23550 Abstract…
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Journal Club, Rebecca E.Lane et al, Analysis of exosome purification methods using a model liposome system and tunable resistive pulse sensing
Motivation and Background: Isolation of exosomes is a critical step for downstream diagnosis and therapeutic applications. Table 1 is a comparison table for four different protocols of exosomes isolation which have been designed to utilize physical properties of these vesicles.…
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Predicting the failure of silicon nanomembranes in burst pressure studies
After years of on again/ off again work – dating all the way back to the earliest days of the NRG – we have finally submitted a manuscript on membrane mechanics. The story of this manuscript is a remarkable one:…
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ECMO: Results and Conclusions
After several months of modeling, experimenting, thinking and rethinking, it’s finally time to talk about the conclusions we can draw about nanomembrane ECMO. These conclusions take into account only blood oxygenation, and not blood decarboxylation, which is a different therapy…
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Getting (lots of) DNA though NPN filters
Warning: the following post contains spoilers for Vincent’s talk at Rochester on Monday. Since the last update, I have tried to get the gold nanoparticles working properly, with no luck so far. The zeta potential we measured in KCl was…
Posted in NRG
Experimental Validation of the ECMO Model
Two of my recent posts have dealt with my efforts to model ECMO and to experimentally validate this model. This post will describe the key change I made to my experimental system since my last post and describe my efforts…
Posted in NRG
