Residual matrix from different separation techniques impacts exosome biological activity (PDF) Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta & Paolo Bergese. Scientific Reports 6, Article number: 23550 (2016) doi:10.1038/srep23550 Abstract…

Motivation and Background: Isolation of exosomes is a critical step for downstream diagnosis and therapeutic applications. Table 1 is a comparison table for four different protocols of exosomes isolation which have been designed to utilize physical properties of these vesicles.…

After years of on again/ off again work – dating all the way back to the earliest days of the NRG – we have finally submitted a manuscript on membrane mechanics. The story of this manuscript is a remarkable one:…

After several months of modeling, experimenting, thinking and rethinking, it’s finally time to talk about the conclusions we can draw about nanomembrane ECMO. These conclusions take into account only blood oxygenation, and not blood decarboxylation, which is a different therapy…

Warning: the following post contains spoilers for Vincent’s talk at Rochester on Monday. Since the last update, I have tried to get the gold nanoparticles working properly, with no luck so far. The zeta potential we measured in KCl was…

Two of my recent posts have dealt with my efforts to model ECMO and to experimentally validate this model. This post will describe the key change I made to my experimental system since my last post and describe my efforts…

Overview of Experiment This experiment examined the behavior of the sieving coefficient graph under mixed particle size conditions, where one particle was significantly greater than the average pore diameter, and therefore acted as a “plug” for the larger pores. With…

To image the blood side (in-channel) side of the membranes, I transferred the membranes of a HD chip onto another wafer segment (SiN wafer: 4534) The transfer wasn’t clean but I got some good images. These membranes were exposed to…

The laboratory has been using our Langer peristaltic pumps for flow experiments in devices with saturated cell culture. A problem we’ve been having with microporous membranes was the shearing of the cells off it, which was attributed to the deflection…

This data comes from our collaborators in Switzerland who are working on a BBB culture model using NPN. In this study they are looking at the effects of co-culture on barrier function. The co-culture is with pericytes and a vascular…