Category: NRG

Journal Club for June 6. Voting is Now Open.

Voting is open until NRG tomorrow. We’ll critically discuss the winner and summarize the 2nd place paper. [yop_poll id=”3″]

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Mechanical memory and dosing influence stem cell fate

This is a recent paper in Nature Materials that shows stem cells are influenced by prior culture on varying stiffness substrates. This means that when cells are induced towards adipogenic or osteogenic lineages, their prior culture history on a stiff or soft

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Variance in Streaming Potential Measurements (Journal Club)

This paper (Variance of Streaming Potential) examines the sources of error in zetapotential measurements. Jess and Zydney both cite it in their EO papers as a source of experimental procedure – in particular this paper found that waiting for the

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Microfluidic Dose Response Assay

Sugiura 2010 (Anal. Chem) This paper details a PDMS microfluidic system to perform dose response assays  (what I’m hoping to achieve over this summer). They use serial dilutions to get separate trials spanning six orders of magnitude, showing an optimal dosage

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An ultrasensitive molecularly-imprinted human cardiac troponin sensor

This paper touches on two thing of current interest to me: Electrical Impedance Spectroscopy (EIS) and troponin detection. Tropinin detection has been identified as a useful biomarker for a chip based blood analyzer. Tropinin is a plasma biomarker that correlates

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Articles for (Jun/06/2014)

Hi all, i have to present for a biomechanics journal club meet next Friday (Jun/06/2014) and figured that i might as well double-dip the presentation.   Currently i am leaning forward to present this article: Takano (Futai) microfld nanofld 2012

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Incorporating a ECM hydrogel into an in vitro microvasculature system – Part III

During the last weeks of my rotation, Tejas and I attempted to study neutrophil migration in the following setup, using fMLP as a chemoattractant: In order to do this, we permanently bonded a 1 mm PDMS gasket to the bottom and

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TEOS film stress

Kelly & I deposited 100 & 300 nm thick SiO2 films on bare wafers with the P5000 PE-CVD tool at RIT last week.  The wafers were then annealed according to JP’s recipe overnight to stabilize the TEOS layer film stress. 

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Cell growth in micovasculature mimetic device

I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN

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Creating Endothelial Cells from Adipose-Derived Stem Cells

In conducting experiments to address reviewer comments, we again created endothelial-like cells from adipose derived stem cells (ADSCs). As in the past, the vast majority of ADSCs express some CD31 (PECAM-1) after culture in endothelial media (M200) supplemented with 50ng/mL

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