Category: NRG

NSF/GOALI- URNano-Fisher projects/goals

The overarching goal of this collaboration is to expose students and faculty at primarily-undegraduate institutions to the technology & expertise available at the University of Rochester.  We would like to make this kind of experience a recurring theme at URNano,…

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Testing CMFDA for Measuring Proliferation

The goal of the experiment was to use a fluorescent dye to label live cells in order to measure proliferation over several days.  To avoid cell toxicity, the intensity of the fluorescence was reduced to ~1.5%.  Human umbilical vein endothelial…

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NSF/GOALI supplemental award: Bringing Nano to Undergraduates @ Fisher

Hello NRG! Thanks to Jim McGrath and NSF, St. John Fisher College students and myself will have the opportunity to conduct research at URNano and the McGrath Lab this summer.  I am an Assistant Professor at Fisher in the Biology…

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BSA concentration has extreme and significant effect on rate of diffusion through membrane

For a while now I’ve been running diffusion experiments using bacterial alkaline phosphatase (bAP) as my solute of interest in a solution of 50mM tris buffered saline (TBS) and 20 ug/mL BSA. Initially, the BSA was included as a way…

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Journal Club for June 6. Voting is Now Open.

Voting is open until NRG tomorrow. We’ll critically discuss the winner and summarize the 2nd place paper. [yop_poll id=”3″]

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Mechanical memory and dosing influence stem cell fate

This is a recent paper in Nature Materials that shows stem cells are influenced by prior culture on varying stiffness substrates. This means that when cells are induced towards adipogenic or osteogenic lineages, their prior culture history on a stiff or soft…

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Variance in Streaming Potential Measurements (Journal Club)

This paper (Variance of Streaming Potential) examines the sources of error in zetapotential measurements. Jess and Zydney both cite it in their EO papers as a source of experimental procedure – in particular this paper found that waiting for the…

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Microfluidic Dose Response Assay

Sugiura 2010 (Anal. Chem) This paper details a PDMS microfluidic system to perform dose response assays  (what I’m hoping to achieve over this summer). They use serial dilutions to get separate trials spanning six orders of magnitude, showing an optimal dosage…

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An ultrasensitive molecularly-imprinted human cardiac troponin sensor

This paper touches on two thing of current interest to me: Electrical Impedance Spectroscopy (EIS) and troponin detection. Tropinin detection has been identified as a useful biomarker for a chip based blood analyzer. Tropinin is a plasma biomarker that correlates…

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Articles for (Jun/06/2014)

Hi all, i have to present for a biomechanics journal club meet next Friday (Jun/06/2014) and figured that i might as well double-dip the presentation.   Currently i am leaning forward to present this article: Takano (Futai) microfld nanofld 2012…

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