We performed four hours of sham dialysis, using two non-porous nitride membranes. The set-up is described in an earlier post. Blood samples were taken when the animal was received, after one week of the adenine containing diet, then at 1 hour…
Category: NRG
HUVEC spreading on various microporous SiO2 membranes
This is part of our work studying how cells adhere, spread and proliferate on varying pore size/porosity SiO2 membranes. This post will be a qualitative look at how HUVEC spread over membranes by staining for actin filaments. In a future…
IV curve analysis of 20 nm NPN
These chips were 2.9 mm TEM grid with a single 25 um window. 20 nm thick NPN is covered with 100 nm TEOS with a 2 um opening to expose ~1000 pores. Wafer number 2176. Following are IV curve analyses…
Posted in NRG
The New Concentration Polarization Model Turns Out To Be Difficult To Implement In Matlab
This is part three of a series of posts on constructing a mathematical model of the movement of gold through a porous membrane. In part one, I implemented William Deen’s mathematical model of the electrostatic and steric effects that contribute…
Posted in NRG
Reduced diffusion distance increases rate of solute transfer through membranes
See my previous post for background information. It has been established that diffusion of small particles (diameter < roughly 1/3 pore diameter) across ultrathin membranes is mediated almost entirely by the ability of the particle to discover a pore, rather…
Posted in NRG
Culture Attempt #1 on custom PLA transwells for SEPCONS
Meghan and I continued to experiment with our PLA printed transwells. We came up with a protocol that has some cells growing on pnc-Si (albeit not to confluence yet), and less so on nonporous Silicon nitride. In the gallery below…
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Incorporating Concentration Polarization into Our Convective Sieving Model
Prior to my qualifying exam about a month ago, I put together a simple model of transport (detailed in this previous post) across a nanomembrane that incorporated electrostatics as well as terms describing both convective and diffusive transport across the…
Posted in NRG
SEM of Nanoparticles and NPN
Below are various SEMs of NPN membranes. (All samples were coated with metal by using the Denton Ag sputterer for 120 s) These were used samples that have been sitting out in the lab for weeks. I don’t remember the exact…
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Diffusion through nanoporous membranes as a function of well height
For a while now I’ve been working on developing and testing the practicality of using nanoporous membranes as filters for highly sensitive assays. Traditional filter-based assays are restricted in their speed by both the time it takes for a particle…
Posted in NRG
Native Oxide Etching for Micropores SiO2 Membranes
Bob and I are trying to determine which wet etch would be appropriate to use to remove the native oxide inside the pores before membrane liftoff. The issue is when removing the native oxide, we will remove part of the…
Posted in NRG
