During the last weeks of my rotation, Tejas and I attempted to study neutrophil migration in the following setup, using fMLP as a chemoattractant: In order to do this, we permanently bonded a 1 mm PDMS gasket to the bottom and…
Category: NRG
TEOS film stress
Kelly & I deposited 100 & 300 nm thick SiO2 films on bare wafers with the P5000 PE-CVD tool at RIT last week. The wafers were then annealed according to JP’s recipe overnight to stabilize the TEOS layer film stress. …
Posted in NRG
Cell growth in micovasculature mimetic device
I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN…
Posted in NRG Tagged with: cell culture, closed flow system, devices, microporous nitride, tissue barrier models
Creating Endothelial Cells from Adipose-Derived Stem Cells
In conducting experiments to address reviewer comments, we again created endothelial-like cells from adipose derived stem cells (ADSCs). As in the past, the vast majority of ADSCs express some CD31 (PECAM-1) after culture in endothelial media (M200) supplemented with 50ng/mL…
Sham Dialysis Study
We performed four hours of sham dialysis, using two non-porous nitride membranes. The set-up is described in an earlier post. Blood samples were taken when the animal was received, after one week of the adenine containing diet, then at 1 hour…
HUVEC spreading on various microporous SiO2 membranes
This is part of our work studying how cells adhere, spread and proliferate on varying pore size/porosity SiO2 membranes. This post will be a qualitative look at how HUVEC spread over membranes by staining for actin filaments. In a future…
IV curve analysis of 20 nm NPN
These chips were 2.9 mm TEM grid with a single 25 um window. 20 nm thick NPN is covered with 100 nm TEOS with a 2 um opening to expose ~1000 pores. Wafer number 2176. Following are IV curve analyses…
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The New Concentration Polarization Model Turns Out To Be Difficult To Implement In Matlab
This is part three of a series of posts on constructing a mathematical model of the movement of gold through a porous membrane. In part one, I implemented William Deen’s mathematical model of the electrostatic and steric effects that contribute…
Posted in NRG
Reduced diffusion distance increases rate of solute transfer through membranes
See my previous post for background information. It has been established that diffusion of small particles (diameter < roughly 1/3 pore diameter) across ultrathin membranes is mediated almost entirely by the ability of the particle to discover a pore, rather…
Posted in NRG
Culture Attempt #1 on custom PLA transwells for SEPCONS
Meghan and I continued to experiment with our PLA printed transwells. We came up with a protocol that has some cells growing on pnc-Si (albeit not to confluence yet), and less so on nonporous Silicon nitride. In the gallery below…
Posted in NRG
