Pint Silicon Supercapacitor Tejas sent me an interesting article about Silicon-based supercapacitors. I have made a previous post on potential uses for our membranes as separators in EDLC (electrical double layer capacitors), and one of the problems I posited…
Category: NRG
Paper Summary – Surface Engineered Porous Silicon for Stable, High Performance Electrochemical Super Capacitors
Posted in NRG
HUVECs Contracting on Nonporous SiO2 120nm Membranes
While performing an experiment to study HUVEC growth rates on various substrates, I observed an interesting phenomena on the oxide membranes. I was using 120nm, nonporous, 600C annealed SiO2 membranes in this trial. The cells were observed at least once…
Posted in NRG
Modeling EOF and Electric Field Gradient at a Microporous Membrane
I used COMSOL for finite element analysis (FEA) modeling of EOF and DEP at a microporous membrane in a PDMS microfluidic channel. Effects of two parameters were determined: Membrane thickness, and spacing between pores. The modeled membranes had a 25×25…
Adipose Stem Cells on Nitride LiftOff Membranes (Part 1)
We have successfully plated adipose derived stem cells (ADSC) on microporous silicon nitride (SiN) membranes. Cells plated on nanoporous SiN appeared fine at time of seeding, but membranes were broken at 2 hours – possibly due to media washing or…
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PDMS cell channels
Michael and I have been working to create cell channels of varying widths and heights to test out a concept used in a paper we recently read as a group. This work claims to see cell cords in 12 hours,…
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Drifting and Biasing Potentials in the Electrodes Make Streaming Potential Measurements Difficult
A few weeks back I had finished my streaming potential/electro-osmotic pump setup. It looks like this: For streaming potential measurements, we pressurize a tube filled with 100mM KCl that connects to the membrane and measure the voltage drop across the…
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Fluorescence Focal Test – 300nm Microporous Oxide Membranes Do Not Wrinkle or Sag After Autoclaving
Method: Oxide membranes in some conditions have been shown to wrinkle over time. To study this, three different varieties of SiO2 membranes were observed (1000C nonporous 120nm SiO2, 150W deposited microporous low-density 300nm SiO2, and 100W deposited microporous low-density300nm SiO2).…
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PEG Mono-layer Detection on Crosslinked Oxide Chips
Below are the results of my recent crosslinking efforts. PEG with 1.5% crosslinker was exposed to UV light at varying times through a bar printer/transparency mask measuring 100/200. In theory this corresponds to 100 micron PEG bars and 200 micron…
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Co-Culture of ADSCs and HUVECs
Scope: Adipose derived stem cells were seeded onto Corning 3um tracketch membranes, human endothelial derived stem cells were seeded onto the membranes approximately five hours later. Numerous control wells were also seeded with ADSCs both on membranes and on the…
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TEOS Membrane Co-Culture Devices
Over the past week Andrea and I designed a new co-culture support structure for use with SiMPore’s 5.5 mm square membranes. The devices are built for use in standard 24-well plates and should prove useful to anyone wanting to do…
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