A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic
Category: NRG
Review of ACKD nanotechnology articles (Vol 20, issue 6, Nov 2013)
This is a cursory review of the nonotechnology articles appearing along with our paper in the Journal “Advances in Chronic Kidney Disease” Most of these are reviews and most deal with nanoparticles for either imaging or therapy. One does discuss…
Co-Culture on SiO2 3um Low Density Membranes
Scope: Human umbilical vein endothelial cells (HUVEC) and adipose derived stem cells (ADSC) were co-cultured on SiO2 low density 3um pore membranes. The ADSCs were initially seeded onto the membranes, at 6 hours the membranes were flipped over and placed…
Posted in NRG
NSF Proposal: Tunable dielectrophoretic separation of nanoparticles with ultrathin microporous silicon membranes
Blanca, Tom, Aytug and I are working on a proposal to NSF that will be submitted Oct.29th to the Chem. & Bio. Separations Program of the CBET division. This is a joint 3-year project between Blanca’s and Tom’s labs. Basically,…
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Paper Summary – Surface Engineered Porous Silicon for Stable, High Performance Electrochemical Super Capacitors
Pint Silicon Supercapacitor Tejas sent me an interesting article about Silicon-based supercapacitors. I have made a previous post on potential uses for our membranes as separators in EDLC (electrical double layer capacitors), and one of the problems I posited…
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HUVECs Contracting on Nonporous SiO2 120nm Membranes
While performing an experiment to study HUVEC growth rates on various substrates, I observed an interesting phenomena on the oxide membranes. I was using 120nm, nonporous, 600C annealed SiO2 membranes in this trial. The cells were observed at least once…
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Modeling EOF and Electric Field Gradient at a Microporous Membrane
I used COMSOL for finite element analysis (FEA) modeling of EOF and DEP at a microporous membrane in a PDMS microfluidic channel. Effects of two parameters were determined: Membrane thickness, and spacing between pores. The modeled membranes had a 25×25…
Adipose Stem Cells on Nitride LiftOff Membranes (Part 1)
We have successfully plated adipose derived stem cells (ADSC) on microporous silicon nitride (SiN) membranes. Cells plated on nanoporous SiN appeared fine at time of seeding, but membranes were broken at 2 hours – possibly due to media washing or…
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PDMS cell channels
Michael and I have been working to create cell channels of varying widths and heights to test out a concept used in a paper we recently read as a group. This work claims to see cell cords in 12 hours,…
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Drifting and Biasing Potentials in the Electrodes Make Streaming Potential Measurements Difficult
A few weeks back I had finished my streaming potential/electro-osmotic pump setup. It looks like this: For streaming potential measurements, we pressurize a tube filled with 100mM KCl that connects to the membrane and measure the voltage drop across the…
Posted in NRG
