I etched 12 pncSi chips and gave it to Chris for EA treatment. I wanted to test 3 storage conditions: gel pack, vacuum container, and water, for total of 4 days including the day of coating as day zero. Unfortunately…
Category: NRG
E-beam Deposition of Ag, Cold ALD Runs, and Sepcon Assembly For Making a Nanofluidic Transistor
Last week I conducted a variety of experiments to try to make a nanofluidic transistor (a device described in detail in this previous journal club post). I did some light optimization of the E-beam deposition of Ag films over a 3nm…
Posted in NRG Tagged with: 100C ALD, 5 nm gold, Ag film, Dynamically Modulate Surface Charge, Nanofluidic transistor
PEGylation of Coverslips and Chips
I’ve taken fluorescence measurements with the fluorescence microscope on PEGylated and unPEGylated glass coverslips and SiN chips. Six coverslips and six chips were prepared. Samples were treated exactly the same, save for the fact that half the samples had PEG…
Posted in NRG
Fluorosphere separations with SiN-NP SEPCONS
Karl and I attempted once again to filter particles with the SiN-NP 5 slot SEPCON chips. (wafer # 1019, 27% porosity, avg. pore dia. – 54 nm in the center, 30%, 61 nm at the perimeter, histograms below) We…
Posted in NRG
TEOS and pnc-Si Discoloration Assay
Over the last week, I’ve run a discoloration assay similar to one run a while back (Barrett’s post here). It was demonstrated in a subsequent paper (Agrawal2010) that annealing the pnc-Si films prevents discoloration of the membrane, which he tied to…
Posted in NRG
Initial Testing of HUVECs w/ CMFDA
When the HUVECs were first brought up, the first test for me to perform was to see how they reacted to CMFDA and repeated fluorescent excitations. Based on the implications from previous research (summarized here), there is the possibility of…
Posted in NRG
Mr. Smith goes to Boise
On June 10th, 11th, and 12th I was in Boise, ID for the North American Membrane Society (NAMS) annual meeting. While there, I presented Jess’s work on the EO pump in a poster session. I got the chance to meet Andrew…
Posted in NRG
1st order approx of transmembrane pressure in Dean's chip-in-a-beaker system
Hi all, During last NRG meeting some questions are raised on what is the transmembrane pressure in Dean’s chip-in-a-beaker dialysis system and what is the direction of flow of the whole system. i gave them some thought and proposed the following…
Posted in NRG
Trouble Making Non-Adsorptive CytoVu Assemblies
Last week I made a post about the troubles that a toluene-based PEGylation process presents to the PDMS in the CytoVu assembly. It was suggested that this issue might be overcome by replacing toluene with ethanol in the process, but…
Posted in NRG
CMFDA Dye Potentially Harms Cells
Invitrogen offers a number of stains and dyes for use in tracking cells and fluorescent imaging of tissue culture. One prevalent live stain is called CMFDA (5-Chloromethylfluorescein Diacetate), a green fluorescent molecular probe. This stain is utilized for tracking cells…
Posted in NRG
