Over the last week, I’ve run a discoloration assay similar to one run a while back (Barrett’s post here). It was demonstrated in a subsequent paper (Agrawal2010) that annealing the pnc-Si films prevents discoloration of the membrane, which he tied to…
Category: NRG
Initial Testing of HUVECs w/ CMFDA
When the HUVECs were first brought up, the first test for me to perform was to see how they reacted to CMFDA and repeated fluorescent excitations. Based on the implications from previous research (summarized here), there is the possibility of…
Posted in NRG
Mr. Smith goes to Boise
On June 10th, 11th, and 12th I was in Boise, ID for the North American Membrane Society (NAMS) annual meeting. While there, I presented Jess’s work on the EO pump in a poster session. I got the chance to meet Andrew…
Posted in NRG
1st order approx of transmembrane pressure in Dean's chip-in-a-beaker system
Hi all, During last NRG meeting some questions are raised on what is the transmembrane pressure in Dean’s chip-in-a-beaker dialysis system and what is the direction of flow of the whole system. i gave them some thought and proposed the following…
Posted in NRG
Trouble Making Non-Adsorptive CytoVu Assemblies
Last week I made a post about the troubles that a toluene-based PEGylation process presents to the PDMS in the CytoVu assembly. It was suggested that this issue might be overcome by replacing toluene with ethanol in the process, but…
Posted in NRG
CMFDA Dye Potentially Harms Cells
Invitrogen offers a number of stains and dyes for use in tracking cells and fluorescent imaging of tissue culture. One prevalent live stain is called CMFDA (5-Chloromethylfluorescein Diacetate), a green fluorescent molecular probe. This stain is utilized for tracking cells…
Posted in NRG
PEG microarray on oxide membrane
We have had success cross-linking PEG to TEOS membranes pretreated with TPM to aid in adhesion. This was done first on nonporous membranes, and then porous. We used 50% PEG at 1.5% cross-linker, which cross-linked at 8000ms. For comparison, 100%…
Posted in NRG
PLA Plastic Acidifies DMEM + 10% FBS
I have been looking into PLA (Poly Lactic Acid) plastic for use in our microfluidics. PLA is a commonly used material in 3-d printers, so it would be great if we could print out our microchannels for cell-culture devices.…
Posted in NRG
SiN-NP with varied RIE times
We used 4″ wafers with 40 nm pnc-Si on 50 nm RV SiN (RTP 1050 C, 100 C/s, 60 s). JP ran a series of RIE times from 10 to 50 s with 10 s intervals. (We had initially planned on…
Posted in NRG
