Silver chloride electrodes are useful for microfluidic devices since they can generate current below one volt. This allows them to function without fear of electrolysis confounding experimental results by way of gaseous clogging. Several different methods for coating silver mesh…
Category: NRG
EO at 1 mM vs 100 mM salts & the role of a meniscus in pressure measurements
Presentation from yesterday: PPT, PDF
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Fluorescence on Single-Well CytoVu – Phalloidin & DAPI
In this trial, bEnd.3 cells were seeded onto three single-well CytoVu devices with SiO2 membranes coated with a 1% Geltrex coating. After ~27 hours, the cells were fixed and permeabilized (the fixing may be only partial though, as the solution…
Posted in NRG
Gold NP separations with SiN-NP
Karl and attempted to pass 10, 20, 40 and 50 nm Au NPs through samples from the center of wafer # 1019 (avg por dia 50-60 nm, porosity ~27%). The bottles of stock Au from BBI solutions do not have…
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Proposed Dielectrophoresis (DEP) Tests w/ Microporous SiOx Membranes
The Gaborski lab has been working with Blanca’s lab performing some scoping tests w/ SiN and SiOx microporous membranes. The aim is obtain proof of concept results regarding the efficacy of using a microporous membrane to trap sub-micron particles with…
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Evidence of ElectroOsmotic Flow in Nano-Membrane Devices
Fluorescent beads were employed to assess electroosmotic flow in various devices. The beads were 200 nm in size and were negatively charged (on account of being carboxylated polystyrene). These beads were suspended in a 0.2 mM K2HPO4 buffer. They were…
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n-Dodecyl β-D-Maltoside Coating on PDMS
Today I performed a coating of n-Dodecyl β-D-Maltoside (“DDM”) on PDMS in an attempt to block protein adsorption (reference). The reference describes two different procedures, one in which the protein being incubated on the PDMS surface is in solution with…
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New laptop for the TECAN and DLS is finally hooked up
Over the past few days Josh and I installed windows 7, windows office suite, Malvern’s Zetasizer software (available online), Tecan’s I-control software (available in the 3-ring binder in the glass cabinet above the machines), and chrome onto the new(er) laptop…
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Permeance and Hydraulic Permeability of ~500 nm pores in 5-slot chips
Permeance Testing Sepcons were built with 500 nm 5-slot chips. Permeance tests were performed on Samples A-C. Nitrogen gas was pushed through the membrane at a given pressure, and the height on the flow meter tube (green graduated column pictured…
Comparative Single-Well CytoVu Images
After experimenting with some different coatings and membrane types in single-well CytoVu devices, here are some representative images of the results: SiN (Nitride) membrane with a Geltrex non-gel coating @ 44hr: 10x: 20x: 40x: SiO2 (Oxide) membrane with a Geltrex…
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