This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position…
Category: NRG
10dynes/cm^2 Aline bottom channel
AlineFlowBottomChannel
hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 2
Introduction This post highlights results from my second attempt to differentiate hiPSCs into endothelial progenitor cells EECM-BMEC-like cells in our lab. For details on methodology, see Nishihara et al., FASEB, 2020 and my previous post. Methods Cells were seeded at 70,000 and 100,000…
Posted in NRG
10 dynes/cm^2 bottom channel Aline
AlineFlowBottomChannelSup AlineFlowBottomChannel
Basement Membrane Disruption by Tumor Necrosis Factor-α in the μSiM-hNVU
Note: a postscript regarding an assay to determine the activity of our MMP-2/MMP-9 inhibitor was added to this post on June 14, 2021. Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability…
Electrospinning aligned polymeric fibers on parylene stencils: cell culture and pll-g-PEG coating update
A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given…
Posted in NRG
Bad to the Bone: Hydroxyapatite Coating Silicon Nanomembranes
In support of the bone infection studies done in the Schwarz lab at URMC, I have recently been tasked with coordinating a new collaboration with Dr. Yates lab in the Chemical Engineering department. The goal of this collaboration is to…
Posted in NRG
ALine for EV Capture
ALine Capture Device In this post I will go over some of my recent work that is a continuation of Kilean’s research concerning extracellular vesicle (EV) capture and characterization. In the past, this was done using a tangential flow filtration…
Posted in NRG
Flow Cell/µSiM Testing – Push versus Pull Flow Modes
Introduction Static endothelial cell cultures have been routinely performed and optimized on the µSiM platform for multiple membrane and cell types. In order to increase the physiological relevance of our device with tissue vasculature mimetics, we need to incorporate fluid…
Human brain vascular pericyte viability in endothelial media
Introduction Mural cells, including smooth muscle cells and pericytes, are important for supporting endothelial cells in the vasculature [1]. They play a role in angiogenesis, and contribute to the development of the vascular basement membrane that provides structure for blood…
