In support of the bone infection studies done in the Schwarz lab at URMC, I have recently been tasked with coordinating a new collaboration with Dr. Yates lab in the Chemical Engineering department. The goal of this collaboration is to…
Category: NRG
ALine for EV Capture
ALine Capture Device In this post I will go over some of my recent work that is a continuation of Kilean’s research concerning extracellular vesicle (EV) capture and characterization. In the past, this was done using a tangential flow filtration…
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Flow Cell/µSiM Testing – Push versus Pull Flow Modes
Introduction Static endothelial cell cultures have been routinely performed and optimized on the µSiM platform for multiple membrane and cell types. In order to increase the physiological relevance of our device with tissue vasculature mimetics, we need to incorporate fluid…
Human brain vascular pericyte viability in endothelial media
Introduction Mural cells, including smooth muscle cells and pericytes, are important for supporting endothelial cells in the vasculature [1]. They play a role in angiogenesis, and contribute to the development of the vascular basement membrane that provides structure for blood…
hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 1
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and…
Electron Beam Lithography Microchannel Fabrication
This post is a continuation of project found in Fabricating 0.5 um Channels in Microscope Slides for Staph Invasion Assays. Just to review, after the development of μSiM-CA (Canalicular Array; see Masters et al., Nanomedicine: Nanotechnology, Biology, and Medicine 2019),…
SPIE Photonics West BiOS Conference Presentation: Rapid detection of intact SARS-CoV-2 viral particles using silicon nanomembranes
This year we were invited to submit a manuscript and presentation to the SPIE Photonics West BiOS Conference. We submitted both on the work we have done on the uSIM-DX virus sensor platform. Below I have attached the presentation which…
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Electrospinning aligned polymeric fibers on parylene stencils
The aim of this work was to electrospin aligned polymeric fibers on parylene stencils as means to characterize the critical necessary total area and necessary opening sizes to elicit endothelial cell response in the form of cell alignment among other…
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Modification of parylene C/N membranes for altered cell-substrate interactions
The motivation of this work by Daniela Lincoln (master’s student) was to investigate the feasibility of using different variants of parylene (C and N) for barrier studies and to look deeper into the implications of plasma surface modification for cell-substrate…
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Cell-substrate interaction modeling
The idea of this project started when we were writing a mini-review about cell-substrate interaction. Although most of studies of cellular responses to porous membranes have pointed towards a multiphasic response with the same trend of changes with respect to…
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