The initial purpose of exploring formlabs SLA 3D printer for a collaboration with Dr. Nicola Marchi (link to previous post). The purpose of the this work was gaining the ability to use a simple membrane on 3D printed platform…
Category: NRG
The End (Regretfully Without Commentary from Far Away Tropical Islands…)
Hello All, I wished to be writing this from some far away tropical resort, as I was told PhD students of the past used to do before they defended their theses. Instead, this comes straight from the ROC homeland, deep…
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Measuring changes in endothelial basement membrane integrity
Introduction Inflammatory diseases have the potential to disrupt blood vessel permeability. This has serious ramifications for the blood-brain barrier (BBB), which is the interface between the vasculature and the brain. A healthy BBB allows essential nutrients to pass through while…
Maintaining Polarized Inflammation and ICAM-1 mAb Blocking – FINAL Polarized TNF-alpha Revision Experiments
Introduction Read the manuscript here: Integrative Biology Publication Please refer to the linked manuscript for a detailed introduction. In short, we have been interested in characterizing the role of endothelial cell (EC) apicobasal polarity in inflammation. While usually reserved for…
Creating Unwanted Vacuoles in Endothelial Cells With Flow – Mossu et al – unpublished
Vacuoles with pulling flow vs pushing flow This video shows an example of the first trials with the uSim flow cell (Tejas’ early versions) when creating laminar flow over a monolayer of BLECs – see Mossu et al, JCBFM 2018…
Microengineered three-dimensional collagen fiber landscapes with independently tunable anisotropy and directionality
Link to BioRxiv paper The extracellular matrix in vivo is composed of fibers arranged with varying degrees of alignment in different directions. The extent of alignment (anisotropy) and the directionality of fibers are an indicator of breast cancer metastasis, influence…
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Peristaltic pump volumetric flow rate experiment data & Calculations
Just wanted to drop this data here in case it may be useful for others in the lab. I calculated the volumetric flow rates for one of the peristaltic pumps used in the lab for the flow circuit in cell-culture.…
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HUVEC polarity to TGF-β1 treatment
Introduction: Transforming Growth Factor -β1 (TGF-β1) is a key player in the induction and progression of fibrosis in many tissues. This factor has also been established as a regulator in the development of fibrotic diseases for its involvement of different…
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Human Umbilical Vein Endothelial Cell Shear on Manufactured Hybrid (Dual-Scale) Membranes
Introduction In our labs previously work, difficulties in growing endothelial cells (ECs) under shear flow lead to the exploration of novel membrane technologies that permit firm EC attachment, without compromising membrane permeability or leukocyte transmigration studies. We ultimately landed on…
SERS nanostructure imaging in NPN and npMgF2, with heat treatments
Over the last few months, I have been trying to reestablish my SERS measurements, and generate a paper based on SERS sensing with our nanomembranes. Previously, I have created magnesium fluoride coated substrates that seem to have better SERS performance…
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