Note: A postscript regarding a gelatin assay to determine MMP-9 activity was added to this post on Feb 25, 2021. Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of undesirable small…
Introduction Previous experiments completed by Hayley Miller investigated the effect of small extracellular vesicles (EVs) collected from Adipose Derived Stem Cells (ADSCs) on wound closure velocity and cell speed. Her previous related post can be found here. It was initially…
Introduction In the Glading Lab we study a rare disease called Cerebral Cavernous Malformations (CCMs). In this disease, brain capillaries develop greatly enlarged lumens and a disrupted blood-brain barrier. A genetic cause of the disease is the presence of a…
This is the second installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position…
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. We are developing a coculture model of the human blood-brain barrier (µSiM-hBBB) to elucidate mechanisms of BBB breakdown…
Introduction Previous work was done on determining physical properties of the glycocalyx of endothelial cells, this was performed on glass inserts using the Asylum AFM. We wanted to continue this testing using the Aline uSim devices in order to…
NOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on…
Background: The human Tendon-on-Chip (hToC) spans collaboration between the Center for Musculoskeletal Research (CMSR) at the UR and the McGrath lab to develop an in vitro platform to study inflammation, fibrosis, and cell-cycle regulation in fibrovascular tendon healing. Interaction between…
Modular µSiM Version 2 (V2) Holds ~30 µl of media in the bottom channel. Designed for cocultures, ELISAs, and other assays that are easier with greater channel media volumes. For more surface area and volume information on mod-µSiM V2, see…
From SiMPore’s Basecamp thread … These are the nanopore characteristics. So between 14% and 20% porosity across the wafer and between 43 nm and 50 nm average pore size. So a little under-sized. I hope T-Cells and Monocytes can get…
