A bunch of samples from W683 (no RTP) were liquid silanized and used in 3D format to track discoloration in comparison to control sample, in DMEM(-FBS) @ 37C in Oven. I used all the cracked chips which broke while clamping…
Category: NRG
Silanization and BSA coating
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in…
Discoloration – loose ends & ground up wafer
There were some ideas being thrown around a couple weeks ago about discoloration results. I tested a couple of them out last week. These experiments were with w615 pieces, in the 37C oven in serum-free DMEM. I tested if vacuum…
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High Resolution TEM at UR – fairly easy
I did some imaging on the URinc TEM today and took the nice image below from wafer SC021. I wanted to see the correlation between highly diffracting (dark) crystals in our standard lower resolution TEM and the lattice fringes observed…
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HUVECs & Microscopy with transwells
Recently, I’ve been frustrated with polycarbonate transwells since they are opaque and I couldn’t see cells on them. So, I bought new transwells from BD (Falcon, PET membrane, 0.4um pores) and decided to test these out. I also wanted to…
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BSA coating on pncSi (3D vs 2D)
I carried out a repetition of BSA coating experiment with both 2D and 3D formats. With 2D, I get the same result as before, but surprisingly, in Sepcon 3D format we observe the opposite to what we see in 2D!…
BSA coating on pncSi (2D)
I coated the pncSi samples from W683 (no RTP) with Bovine Serum Albumin (BSA) in Tris-EDTA buffer. Three different concentrations were used: 1 mg/ml, 5 mg/ml and 10 mg/ml (usual range of BSA conc. used for coating Si surfaces). The…
Sepcon vs Cloning ring II
Repeated the previous experiment with W683 and following images were captured after 5 hours of incubation in DMEM(-FBS), Oven @ 37C. I took Sepcon samples with regular thin o-rings, fat o-ring, without o-ring and w/grease in place of o-rings. Also…
Sepcon vs Cloning ring
I carried out this experiment in order to rule out any effect due to geometry of the setup in our discoloration experiments in Sepcon. I used a cloning ring and mimicked the Sepcon as shown in the figure below. The…
Measuring Gold Separations
Even though we don’t have defect free membranes, I wanted to tweak the gold setup using membranes with pinholes. Previously I was using the small scale diffusion chamber that I use for protein separations. This setup has a retentate of…
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