Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
Category: NRG
15um Bead Flow through Closed Flow Cell
Based on the results obtained yesterday with the closed flow cell we reran another trial today. The closed flow system we tested today was bound using UV Ozone treatment, surprisingly this system created a relatively strong bond despite our resent…
Fibroblasts spreading on glass
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on…
Wafer 620: porosity vs. position
We’ve been assuming that porosity follows a linear relationship with respect to wafer position, but this turns out to be incorrect. I imaged membranes from five points on wafer 620 and found that maximum porosity does not occur at the…
Posted in NRG
concentration curve for PBS + Rhodamine
I mixed made solutions of PBS and Rhodamine with varying Rhodamine concentrations and ran them through the spectrometer. Below is the concentration vs absorption curve I made from it. I blanked the spec with PBS instead of ddH2O since the…
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Progress with Closed Flow Cell
Yesterday Dean, the student from RIT, came over to help us set up our Anodic Bonder. Once we get the proper leads and connections we will be able to perform these bonding trials whenever we want without needing to travel…
Adsorption Quantification VIII
Here is the full updated Adsorption Quantification figure: We were concerned that cellulose appeared to adsorb more than PES when the literature clearly states that it shouldn’t due to its hydrophilicity. We considered that this same hydrophilicity might be part…
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Cell Spreading on pncSi and glass
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time…
Membrane stability in cell culture: various treatment conditions
Four different post-production treatments with the objective of improving membrane stability in culture were run last week on W615, a pinholed wafer laden with the circular features we have been seeing recently. I ran four different conditions for cell culture:…
Rhodamine 6G diffusion through RTP treated and non-treated pncSi (w/o pinholes)
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no…
