I mixed made solutions of PBS and Rhodamine with varying Rhodamine concentrations and ran them through the spectrometer. Below is the concentration vs absorption curve I made from it. I blanked the spec with PBS instead of ddH2O since the…
Category: NRG
Progress with Closed Flow Cell
Yesterday Dean, the student from RIT, came over to help us set up our Anodic Bonder. Once we get the proper leads and connections we will be able to perform these bonding trials whenever we want without needing to travel…
Adsorption Quantification VIII
Here is the full updated Adsorption Quantification figure: We were concerned that cellulose appeared to adsorb more than PES when the literature clearly states that it shouldn’t due to its hydrophilicity. We considered that this same hydrophilicity might be part…
Posted in NRG
Cell Spreading on pncSi and glass
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time…
Membrane stability in cell culture: various treatment conditions
Four different post-production treatments with the objective of improving membrane stability in culture were run last week on W615, a pinholed wafer laden with the circular features we have been seeing recently. I ran four different conditions for cell culture:…
Rhodamine 6G diffusion through RTP treated and non-treated pncSi (w/o pinholes)
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no…
Protein Separations with w612
After doing all the water permeability with this wafer, I saved the samples to do some protein studies. When I write my own paper I will need two wafers to separate protein standards by diffusion and flow to show movable…
Posted in NRG
Update on Anodic Bonding at RIT
Yesterday Henry and I went over to RIT to perform anodic bonding and parylene deposition using the new membrane configuration of W619 (with the etched trenches for our capillary tubing). I just wanted to post a summary of the process…
Wafers 620/621
Today I etched wafer 621, which is the pair to 620. While w620 has a relatively low pinhole density, w621 shows a much higher occurence of defects. Since the front-end processing and thermal treatment was identical for these wafers, I…
Posted in NRG
Water Permeability – Looking at the data
So I talked a bit with Philippe today about the water permeability data, and he suggested that I try to see if there’s a correlation between what the histogram looks like and the experimental permeability’s relation to the theory. I…
Posted in NRG
