We are continuing to work with the Shestopalov lab on surface modifiation of hemodialysis filters to prevent adhesion and activation. Jim recently presented our status and strategy (PDF) to the Technical Development Fund and was awarded the balance of the award,…
Tag: adsorption
Adsorption Test of PEG4, IgG and BSA
Discussion: The BSA may have not been fresh enough to give good data, the difference between background and signal are pretty low. The Rhodamine IgG gave better signals but the PEG4 treated SiN is between the untreated SiN and bare…
Comparitive Functionalization of pnc-Si measured with f-BSA fluorescence intensity
The last of the larger windowless pnc-Si chips were sent to both Adarza and Dr. Shestopalov’s lab for functionalization. Adarza returned chips with PEG-1 and PEG-2 (different rounds of processing) with both 2 hours and 16 hour treatments. They also…
f-BSA Adsorption on Adarza functionalized pnc-Si chips
Chips of pnc-Si were funtionalized at Adarza. They returned four groups of chips. Ethanolamine, Amino PEG-1, Inactivated sLinker, and Amino PEG-2. These were all incubated with PBS and 5-mg/mL f-BSA (2:3) in a humid refrigerated dish. The samples were rinsed…
f-BSA adsorption on Functionalized pnc-Si (Shestopalov Lab)
Katia in Dr. Shestopalov’s Lab functionalized some pnc-Si windowless chips. The process she follows is shown in Figure 1. She provided chips at various stages of the process. “Grignard”, “Diazirine”, and “PEG”. After functionalization, the chips were wetted with…
f-BSA adsorption pnc-Si
Samples of pnc-Si, 5.5 mm x 5.5 mm, were functionalized at Adarza and incubated with fluorescein conjugated BSA. Wells were formed in PDMS to hole fluid above the samples. Samples were wetted with PBS then f-BSA, 5 mg/mL, was added.…
Adsorption of F-BSA on PEGylated pnc-Si, PEGylated Glass, Glass
This study was run to test test the efficacy of PEGylation in blocking BSA adsorption. Three surface types were used, glass, PEGylated glass, and PEGylated pnc-SI. The glass was cleaned in Ethyl Alcohol, dried and UV treated, PEGylated glass was…
