In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at…
Author: Karl Reisig
Desalting: Intact SepCons
Introduction One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Ideal membranes have uniform pore distributions, high mechanical strength, tunable, sharp pore cutoffs, and high porosity. In…
W612 Pinhole Counts: Interesting Features
I am in the process of inspecting each membrane in the newly created wafer 612. First of all, some samples (~10% counted so far) have tears on the membrane edges, shown below. Images were taken at 20x microscope magnification (reflection…
Membrane Stability in Cell Culture
Last week I ran cell culture growth experiments on RTPed (800C 5 minutes in Ar) samples from W567 (single slit 100um x 1000um). For this study I tracked discoloration w/cells, membrane stability w/cells, and the bead assay w/cells to see…
Wafer Testing: W520, 558, 559
I recently received three pinhole-ridden wafers for cell-related experiments. All three have been RTPed at 800C for 5 minutes in argon, which is the typical procedure we have been using for cell culture. The bursts pressures for each wafer are…
Desalting: Continued Work
In continuation of the desalting work, I ran three additional controls: a SepCon with no slits (negative control) and direct addition of 4M NaCl producing final dilutions of 1:400 and 1:600 (these are the experimental final dilutions I have been…
W567 Burst Pressure and Air Permeability
I recently received wafer 567 from Dave for cell culture work. It has many intact membranes but is riddled with pinholes, having only 3 pinhole-free samples. I decided to test them for air permeability and burst pressure, shown below. I…
Membrane Anneal Study: W510X, W600 Testing
I recently tested W510X and W600 described by Dave here for air permeability, rhodamine diffusion, and burst pressure. No air permeability was observed; even a sample with one very small pinhole (W600 800C anneal) only had a permeability of 11…
Desalting: Broken and Pinholed Membranes
Desalting of 4M NaCl in deionized water was performed using our membranes in the SepCon format. I ran the SepCons in the same as way as the previous study, using a low stir rate and floating the SepCons. Once again,…
W509X Burst Pressure and Air Permeability
W509X, with single 100um by 1000um slits, was tested for air permeability and burst pressure. Prior to testing, I inspected all membranes for pinholes.; every sample had pinholes on the membrane perimeter, as shown below. The first two images are…
