Category: NRG

w677 Separation

Here are the results of a protein ladder separation with w677.  If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that…

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Using JMP7 for Membrane Reliability and Performance

JMP7 statistical software was used to perform a Tukey-Kramer analysis on both burst pressure (psi) and air permeability for membranes fabricated on 200um and 400um wafers. This data has been collected from a spreadsheet that is maintained by Karl and…

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DMEM salt/buffer concentrations

I talked about this yesterday in lab meeting, but here is the data.  Since the components of HBSS by themselves did not cause discoloration (HBSS components), I adjusted the concentrations of NaCl, KCl, glucose, Na2HPO4 and NaHCO3 to match that…

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Effect of Active Area on Electroosmosis

Does more active area mean more fluid transported by electroosmosis? To test this I found an ancient wafer (w13) that had different active area geometries. Can someone check and make sure I got these right: Long slit – .1 x…

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Membrane deflection can be characterized using the Veeco Wyco Optical Profiler

An initial experiment for membrane deflection characterization using an optical profiler (Veeco Wyco) has been performed. Below are some of the images taken with the tool: (a image was taken at each 0.5 psi interval): Once we get a pressure…

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Nanotubes on membranes

Jamie and I met with Don Black with an electron microscopist that consults for NaturalNano. In efforts to test out our TEM window grids, he gave us halloysite nanotubes. These are naturally occuring and much larger than the carbon nanotubes…

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Negative Adsorption Assay Retry

This is a retrial of the previously outline negative adsorption assay.  I was a little more careful with cuvettes this time around (there are very significant differences in the plastic cuvettes) and I spread out the same amount of solution…

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Various methods of applying PDMS to NC

During the past week I tried various methods of applying PDMS to nitrocellulose. Below is a table of what the PDMS array looks like on the NC before curing in the over, and after applying 1mg/mL BSA and staining with…

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Counter Flow System Progress

Just wanted to post an update on the creation of a counter flow system using the pncSi chips from wafer 619 (the wafer with the etched trenches for the insertion of capillary tubing). Over the past few weeks Henry and…

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Discoloration – Water soak

This week, I did a quick discoloration experiment based on Chris’s comment at lab meeting.  He wondered if soaking the chips in water for a while would allow potential discoloration-causing contaminants to slowly leech out.  I took 2 chips from…

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