Here’s some data from my electrophoretic trials with polymer membranes. On this chart, both PES and Cellulose fall on the same line.  In my last post I said that PES membranes didn’t seem to pass water, although I found that…

In my last discoloration experiment, I tested buffers with different pKa’s (MES, HEPES, and Tris). I found that only 10 mM HEPES in HBSS caused discoloration, and furthermore, that the HBSS component of this solution was the culprit (post). Fortunately,…

If protein is adsorbing to a surface, it’s leaving the solution.  We can indirectly measure how much protein is leaving the solution, and that may tell us something about how much these surfaces are adsorobing. For my first attempt, I…

To further investigate the mechanism of discoloration, I prepared buffers at different pH’s and measured discoloration and pH over time. Buffers were: 0.1M MES, pH=pKa=6.1; 0.1M Tris-Base, pH=pKa=8.39; 0.1M Tris-HCl, pH=pKa=8.35; 10mM HEPES in 1X HBSS, pH 7.38, where HEPES…

The Burns-Zidney paper uses streaming potential to measure the zeta potential of polymeric membranes.  This means that polymeric membranes should also be capable of performing electro-osmosis, essentially the opposite of streaming potential.  For more on this, I have a knowledge…

I ran a few experiments using the stir cell on the shaker table. Diffusion occurs faster on the shaker, but more air bubbles form around the membrane, thus preventing further passage of rhodamine. The first experiment I ran was with…

Yesterday, Chris, Dave, Graham and I were (exhaustively) trained on the Asylum AFM in Rick Waugh’s lab.  We had an overview of the basic features of the AFM and got some real data, as well. The top panel is a…

Below is an image of the PDMS array on the nitrocellulose membrane. I’m trying to create a stamp that can be applied to the NC membrane, before PDMS is poured on. The idea is that the stamp will be heavy…

Last week in lab meeting, someone wondered if there the time after RTP affects the rate of discoloration. To test this, I RTP’ed chips from w638 in Ar at 800C for 5 minutes. I then followed discoloration in DMEM+10%FBS in…