The Burns-Zidney paper uses streaming potential to measure the zeta potential of polymeric membranes.  This means that polymeric membranes should also be capable of performing electro-osmosis, essentially the opposite of streaming potential.  For more on this, I have a knowledge…

I ran a few experiments using the stir cell on the shaker table. Diffusion occurs faster on the shaker, but more air bubbles form around the membrane, thus preventing further passage of rhodamine. The first experiment I ran was with…

Yesterday, Chris, Dave, Graham and I were (exhaustively) trained on the Asylum AFM in Rick Waugh’s lab.  We had an overview of the basic features of the AFM and got some real data, as well. The top panel is a…

Below is an image of the PDMS array on the nitrocellulose membrane. I’m trying to create a stamp that can be applied to the NC membrane, before PDMS is poured on. The idea is that the stamp will be heavy…

Last week in lab meeting, someone wondered if there the time after RTP affects the rate of discoloration. To test this, I RTP’ed chips from w638 in Ar at 800C for 5 minutes. I then followed discoloration in DMEM+10%FBS in…

Last week, I did a couple experiments based on a suggestion by Tom. The pH of DMEM starts out quite high and then buffers down into physiologic range after some time in the incubator. Tom surmised that a short exposure…

Hi all, For quite some times we have been trying to design a diffusion-based chemotaxis chamber that operates under an environment of 37 degrees Celsius in temperature. One main obstacle that we face is the evaporation of the 2 uL…

Hi all, After experimenting with UV ozone bonding of PDMS to glass, Si, and pnc-Si for the past few weeks with Mike, we have reached the following conclusions: 1. The bonding of PDMS to glass is always stronger than the…