Last week, I did a couple experiments based on a suggestion by Tom. The pH of DMEM starts out quite high and then buffers down into physiologic range after some time in the incubator. Tom surmised that a short exposure…

Hi all, For quite some times we have been trying to design a diffusion-based chemotaxis chamber that operates under an environment of 37 degrees Celsius in temperature. One main obstacle that we face is the evaporation of the 2 uL…

Hi all, After experimenting with UV ozone bonding of PDMS to glass, Si, and pnc-Si for the past few weeks with Mike, we have reached the following conclusions: 1. The bonding of PDMS to glass is always stronger than the…

I used the source meter that Chris brought over and tested the voltage at points across the chamber. I set up the system with 1x PBS at 15V (~10mA) with a pinholed membrane. I tested the voltage drop at the…

I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into…

I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was…

Today I built a small scale electrophoretic cell using the components from Lingyun’s study.  I drilled out his cell and affixed a coverslip to the bottom.  I set up a pinholed membrane just like our standard diffusion assay (with silica…

Last week, five wafers were produced – three had a double 3500 A wet oxide growth/strip and two had a single 3500 A wet oxide growth/strip.  From Jess’ AFM work, an as-is wafer showed a high density of 5-7 nm…