I used the source meter that Chris brought over and tested the voltage at points across the chamber. I set up the system with 1x PBS at 15V (~10mA) with a pinholed membrane. I tested the voltage drop at the…

I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into…

I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was…

Today I built a small scale electrophoretic cell using the components from Lingyun’s study.  I drilled out his cell and affixed a coverslip to the bottom.  I set up a pinholed membrane just like our standard diffusion assay (with silica…

Last week, five wafers were produced – three had a double 3500 A wet oxide growth/strip and two had a single 3500 A wet oxide growth/strip.  From Jess’ AFM work, an as-is wafer showed a high density of 5-7 nm…

I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators.  This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed…

I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell.  In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621.  After that I…

JP handed me a stack of wafers today, and I went over to the Krauss Lab and with the help of Jack Calcines got some AFM images.  I didn’t have much time to play with, so I tried to get…