1. Introduction 1.1 Vascular barriers Functions: Maintain tissue homeostasis, control transport and gas exchange, recruit immune cells during inflammation Figure 1. Schematic illustration of a vasculature barrier in the human body. Endothelial cells as a building element of vasculature barriers…
Category: memBrains
A Modular vascular barrier platform with a removable flow module and high resolution imaging (Publication draft)
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Developing a Distributable µSiM ‘Transwell’-like Platform…Publication Figure Drafts
Introduction Here, I will post figure drafts for the paper that introduces the modular µSiM (m-µSiM). Figures Remaining data to obtain: Occludin staining in IMR90-4 from UniBe in µSiM. We have tried this after 2 day culture in µSiM, which worked…
R61: 12 Month Progress to NIH Slide Decks
R61; 12 month Progress (Rochester) Small Trans-Agency Blood-Brain Interface Program Investigator Meeting – Agenda_FINAL
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IMR90-4 EECM-BMEC-like Cell Analysis in µSiMs
Introduction We are differentiating and validating EECM-BMEC (Extended Endothelial Cell Culture Method-Brain Microvascular Endothelial Cell)-like cells in µSiM devices. See this post and this post for method details and validation in traditional cell culturing platforms. Methods Attempt 1 – Overstimulated cells IMR90-4…
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in situ Permeability Analytical Solution Summary – Unhindered vs Hindered D
Introduction Pages regarding our in situ permeability assay development can be found in these links: Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach) Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 2: Experimental Validation)…
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Competitive ELISA assay to measure the HA shedding from HUVEC monolayer
Competitive ELISA assay to measure the HA shedding from HUVEC monolayer Introduction The endothelial glycocalyx is a dense layer of proteoglycans, glycoproteins, and glycosaminoglycans (GAGs) that helps maintain the integrity of the endothelial cell (EC) barrier.1 The main GAGs are…
COMSOL simulation on sampling method to measure membrane permeability
Background We at Michigan have been aiming to perform sequential small volume sampling coupled with our ultrasensitive digital immunoassay to provide longitudinal data from the uSIM. We have encountered repeatability problems as well as low molecule abundancy with shortly incubated…
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Sampling Method Permeability Assay Optimization
Motivation We have discussed the optimization of our in situ permeability assay at length. This assay relies on confocal microscopy, which may not be accessible to all users of the µSiM. Therefore, we developed an alternative permeability assay in the…
Thinkin’ Secretion in the µSiM
One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289…
Assessing Neutrophil Activation inside a Flow Cell Device with Flow Cytometry
Introduction As part of our continuing evaluation of the flow cell developed by our collaborators at RIT, our next goal is to demonstrate that neutrophils remain inactivated when flown through a first generation device (Figure 1). For this, we studied…
