Introduction Previous work was done on determining physical properties of the glycocalyx of endothelial cells, this was performed on glass inserts using the Asylum AFM. We wanted to continue this testing using the Aline uSim devices in order to…
Category: memBrains
Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach)
NOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on…
Multiplexed digital assay: mouse CCL2(MCP1) CXCL1(KC) panel development
Mouse CCL2/MCP1 and CXCL1/KC pre-equilibrium digital ELISA has been developed with the current dynamic range set to be 4-500pg/ml for CCL2/MCP1 and 8-1000pg/ml for CXCL1/KC. This initial dynamic range was selected by inferring from the ELISA kit reagent working concentration…
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ALine mod-µSiM: Version2
Modular µSiM Version 2 (V2) Holds ~30 µl of media in the bottom channel. Designed for cocultures, ELISAs, and other assays that are easier with greater channel media volumes. For more surface area and volume information on mod-µSiM V2, see…
Flow demo (T cell interaction with human in vitro BBB model under flow)
20210122.flow-movie-demo I attached a link to a short presentation regarding the flow movie setups. The figures and videos will be explained in detail during the meeting on Friday.
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Pore Analysis for Wafer 1383 (Low Porosity Dual Scale)
From SiMPore’s Basecamp thread … These are the nanopore characteristics. So between 14% and 20% porosity across the wafer and between 43 nm and 50 nm average pore size. So a little under-sized. I hope T-Cells and Monocytes can get…
QC on Dual Scale Membrane (lot 1383)_from Bern
We recently received 100 Dual Scale Membrane (lot 1383). As noted in Dan’s post we also had same issues regarding mix up in the labeling. In the gel box, 2 boxes (25 membranes/box) are labeled 0.625% porosity and 2boxes are…
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QC on Dual Scale Membranes (Lot 1383)
Recently we have received dual scale (nanoporous and microporous) membranes from SiMPore at two porosities: 0.625% and 1.25%. These feature 3 µm circular pores (Lot 1383). We routinely perform QC checks on membrane deliveries, so the following is report on…
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Collagen IV is deposited below human umbilical vein endothelial cells
Introduction My previous post focused on attempts to reproducibly generate collagen IV with human umbilical vein endothelial cells (HUVECs) so that we would be able to establish protocols for basement membrane protein detection and degradation measurement. Following my presentation on…
Simulation and PIV results of the flow module
1. Introduction Fluid flow is an influential component of vascular models for conditioning cells and introducing other components such as leukocytes. In the previous post, we described our modular design for integrating a flow module into the uSiM device. Here,…
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