The main difficulty with creating a simulation of the single channel dialysis chip is that the membrane is so very thin (20 nm). This is especially true when compared to its length of 10 mm but also when compared to…
Tag: dialysis
Single Channel 20 nm membrane breakage…
There is a reliability/handling issue with the single slot chips. 0.5 mm x 10 mm slots, 20 nm thick membranes with 40 nm SiN reinforcement. The membranes are easily packaged in an upper fluidic PDMS layer with inlet/outlets and two…
Rapid Prototype of Single Channel Dialysis Device
In order to rapidly test the entire setup for dialysis evaluation, including piezoelectric micro motor and recirculating fluid path, a means of quickly evaluating PDMS designs is needed. A mold was created with a multi-print technique in which printer toner…
Microfluidics in Hemodialysis Project
The purpose of the fluidics in our hemodialysis system is to move the sample to and the retentate away from the membranes. The dialysate (permeate, filtrate) will be handled passively in the initial designs and therefore do not require fluidic…
Attempts at Closed Flow System
I wanted to update everyone on the recent attempts at the formation of the a closed flow system by bonding PDMS to our membrane chips, using capillary tubing for the inlet and outlets. Last week when we tried to preform…
Desalting: Continued Work
In continuation of the desalting work, I ran three additional controls: a SepCon with no slits (negative control) and direct addition of 4M NaCl producing final dilutions of 1:400 and 1:600 (these are the experimental final dilutions I have been…
PDMS-Membrane Bonding Update
This is just an update on the progress being made towards creating a closed flow cell by bonding PDMS to our membrane chips. For those who were not at the meeting on Tuesday we have successfully bonded PDMS to both…
Success with PDMS-Glass Bonding
Yesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room…
Update on using UV Ozone to bond PDMS and Glass
After posting my observed results from yesterday I came in today to find that one of the segment of the PDMS was strongly bound to the glass cover slip, so strong in fact that trying to remove it caused the…
Using UV Ozone to Bond PDMS to Glass
I talked with Jim last Friday about a second possible dialysis system using PDMS bound to a glass cover slip. I made a batch of PDMS and let it cure at room temperature over the past few days. Jim had…
