In this post, pores from four different structures are compared. Those four different structures are nitride/silicon/nitride (NSN), nitride/silicon/oxide (NSO) , oxide/silicon/nitride (OSN) and oxide/silicon/oxide (OSO) . The thicknesses of nitride, oxide and silicon are 30nm, 30nm and 25nm, respectively. Wafers…
Category: NRG
The Effect of Pressure on the Sieving Coefficient of a 20nm membrane with 10nm gold
It was speculated that the rate of diffusion through a charged membrane had a direct relationship with pressure – as pressure increases, the kinetic energy of the charged particles is more likely to overcome the electrostatic repulsion of the membrane,…
Posted in NRG
shear stress reduction via pnc-Si: COMSOL simulation
Hi all! Last time we have presented a theoretical perspective on the shear stress reduction enabled by our shear-free microfluidic system. This time we will show an agreement between the shear-reduction predictions made by the analytical solution and a COMSOL…
Posted in NRG
shear stress reduction via pnc-Si: analytical solution
Hello everyone, presented here is a theoretical perspective on the shear stress reduction enabled by our shear-free microfluidic system. Briefly speaking, the shear-free microfluidic system consists of three main components: a flow compartment, the pnc-Si membrane, and a shear-free compartment.…
Posted in NRG
Rapid Prototype of Single Channel Dialysis Device
In order to rapidly test the entire setup for dialysis evaluation, including piezoelectric micro motor and recirculating fluid path, a means of quickly evaluating PDMS designs is needed. A mold was created with a multi-print technique in which printer toner…
BBB Device Design (updated)
The goal of the blood brain barrier device is to co-culture cells in a closed system, with the apical chamber under flow conditions, while still being able to access either chamber for media changes or to introduce new solutions. Electrodes…
Posted in NRG
Closed System Device
One aspect of the blood brain barrier device is that it be a closed flow system. This would not only better mimic an in vivo environment, but also maintain Sema4D activity, negatively affecting endothelial cell junctions and providing a target…
Posted in NRG
Live/Dead Assay for BBB Device
A quick recap: Vaccinex has asked the lab to design a closed system device with the capability to co-culture on microporous membranes. This device would be used to mimic the blood brain barrier, with endothelial cells cultured on the apical…
Posted in NRG
Barcikowski Collaboration Update
Over the last week or so I’ve taken some lovely SEM pictures with Joe, used the light scatter, and ran some separations on filters I assembled myself. Someone from the Barcikowski lab, Dr. Rehbock, got in touch with me and…
Posted in NRG
Vacuole formation on Nitride
Barrett had discovered that growing endothelial cells on pnc-si or tranwells causes vacuoles to form. The vacuoles on pnc-si formed only on the free standing areas of the membrane where there are open pores. The background on pnc-si chips showed…
