Introduction In prior work by Panteha Torabian and the Michel Lab (RIT’s College of Science), we have seen that treatment of E. coli with different antibiotics can result in changes in the production of bacterial extracellular vesicles (bEVs). In the…
My Talk (PPT; Keynote) Program My Notes
The purpose of this study was to characterize pore sizes on Aquamarijn (Aquamarijn Membranes B.V., the Netherlands) membrane chips. Membranes provided to us by Aquamarijn have a design specification of 450 nm pore size across 14 windows in the chip.…
The SepCon filtration devices represent an innovative housing solution for Silicon Nitride membranes utilized in the filtration of microparticles and nanoparticles. In assessing compatibility, I conducted a test to evaluate the suitability of Aquamarijn’s microsieve membranes with our existing SepCon…
Introduction The modular μSiM is based on a 96-well plate format with a 100 μl open well. While these devices have proven extremely useful for various barrier models including the blood-brain barrier1 and the tendon-vascular interface2, there is a need…
Introduction Alzheimer’s disease (AD) is a neurodegenerative disorder that is characterized by accumulation of amyloid beta (Aβ) peptides and neurofibrillary tangles (NFTs) made of aggregates of the protein tau (1,2). It results in the destruction of neurons and their connections…
Introduction The Slide Body (Figure 1) has been used for a long time to image μSiM devices. This slide body has the same footprint as a traditional microscope slide but is designed to hold up to 2 μSim devices for…
Introduction Sepsis refers to an uncontrolled systemic inflammatory response which can result from bacterial blood infections. We have long been interested in developing a sepsis model using the μSiM-BBB platform because the neuroinflammation stemming from sepsis can cause severe BBB…
The two barrier anatomy of the post-capillary NVU and why it matters At the post-capilliary venule, the blood-brain barrier (BBB) is actually two barriers in series: 1) one created by the pericyte/BMEC layer at the blood interface and 2) one…
Dave Fang posted a poster in 2010 about particles found in DI water. I encountered the same issue during the nano-pocket permeability experiment. He said, “I’ve become increasingly suspicious of membrane clogging during the … permeability experiments.” I conducted an…
