Towards Surface Enhanced Raman Functionalization of Nanopocket membranes Introduction Use of Raman microscopy is used to evaluate the chemical profile of substances. Surface enhanced Raman spectroscopy (SERS) is a signal-boosting techniques where the weak Raman signal is amplified through…
Category: NRG
Rotation Project – Sergio Garcia
Introduction The Ehler-Danlos Syndromes are a group of thirteen heritable connective tissue disorders. These disorders are genetic, and usually involve a mutation in genes that code for components in the collagen matrix (1). Some of the main characteristics in patients…
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EV Enrichment with Izon qEV Column
Introduction This post will describe the preliminary work I have completed using size exclusion chromatography (SEC) for EV enrichment. We are ultimately interested in interrogating serum EV biomarkers using CAD-LB, however, the presence of protein and lipoprotein contaminants reduces the…
Exploring Urinary EV Detection Using CAD-LB
Introduction This post provides an update on a series of experiments conducted with Sam. These experiments were designed to assess the versatility of the CAD-LB platform for detecting EVs in urine samples. Urine is a complex biofluid often used in…
The Base of TraCe: Exploring the Fundamentals of the Etaluma Microscope
For year one of the Trace grant, I will be focusing on the development of a standard operating procedure for the uSiM-CA. To begin this process, I am transferring all of my current operating procedures from the Andor dragonfly confocal…
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Modification of Ultrathin Microporous Silicon Nitride Membrane Stiffness for Tissue Barrier Modeling
Introduction: Developing innovative therapeutics and treatment approaches with improved effectiveness and safety necessitates the use of cell co-culture models and barrier environments that mimic in vivo conditions. These models involve culturing two or more cell types together in vitro, allowing…
George Wadsworth Training Visit
Slide Deck Video Presentation Jove_Video_Comments_Form (GW)
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How to Place Orders in the New NRG Membrane & Component Shop
We are excited to announce our new shop that will allow any Nanomembrane Research Group (NRG) member to easily order membranes and components. This blog will go over the ordering process and provide some useful information on the ordering process.…
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CAD-LB: Biomarker Detection Validation – ICAM-1 HUVEC Stimulation Model
Introduction This post is another update in my series of experiments aiming to both characterize and validate the CAD-LB platform. We have now shifted our focus to validating CAD-LB biomarker detection which will be done using multiple experimental models. The…
Exploring primary human microvascular endothelial cells for μSiM-hBBB modeling
Introduction Sepsis is a maladaptive response to infection with a 25-50% mortality rate and a high incidence of cognitive decline among sepsis survivors.1-6 Because infection originates outside the brain, the factors responsible for cognitive decline in sepsis must get to…
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