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Category: NRG
How to Place Orders in the New NRG Membrane & Component Shop
We are excited to announce our new shop that will allow any Nanomembrane Research Group (NRG) member to easily order membranes and components. This blog will go over the ordering process and provide some useful information on the ordering process.…
Posted in NRG
CAD-LB: Biomarker Detection Validation – ICAM-1 HUVEC Stimulation Model
Introduction This post is another update in my series of experiments aiming to both characterize and validate the CAD-LB platform. We have now shifted our focus to validating CAD-LB biomarker detection which will be done using multiple experimental models. The…
Exploring primary human microvascular endothelial cells for μSiM-hBBB modeling
Introduction Sepsis is a maladaptive response to infection with a 25-50% mortality rate and a high incidence of cognitive decline among sepsis survivors.1-6 Because infection originates outside the brain, the factors responsible for cognitive decline in sepsis must get to…
Posted in NRG
Optical tweezer microrheology: HCIC User Meeting presentation
I recently presented an update at the HCIC User meeting on 11/10/23 detailing our efforts in exploring the use of optical tweezers to perform microrheology in type I/type III collagen hydrogels. These hydrogels are of interest for in vitro models…
Posted in NRG
CAD-LB: Colocalization Detection Guide
Introduction In this post I will review work I have completed to further characterize the CAD-LB platform. In this set of experiments combinations of nanoparticle species were used to define colocalization limitations. More specifically, two nanoparticle species each labeled with…
CAD-IA for traumatic brain injury (TBI) diagnostics
Introduction The glial fibrillary acidic protein (GFAP) is an intermediate protein released by astrocytes and has value as a serum-based biomarker of neurological abnormalities including traumatic brain injury1. Inspired by the CAD-LB method for digital assessment of biomarkers on small…
Posted in NRG
Nano-pocket Membrane Integration in Tangential Flow for Analyte Capture (TFAC) for Precise Size-Based Particle Capture
Introduction: Building upon previous research conducted by the Gaborski and McGrath labs, this poster introduces a novel approach to isolate extracellular vesicles (EVs) based on size. The earlier work involved the development of a tangential flow membrane capture system to…
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Modular assembly of the hToC: An adaptable protocol for interfacing a monolayer with a 3D culture
Introduction: This post presents a comprehensive protocol detailing an in vitro seeding and culturing method tailored for diverse cellular monolayer and tissue interfaces. These interfaces necessitate varying culturing conditions and extended timeframes, facilitated through modular device utilization. The methodology focuses…
Posted in NRG
Investigating the potential of astrocytes as a neuroinflammation markers by assessing their activation state
Introduction Astrocytes, which are the most abundant glial cells and key components of the BBB, are known to play crucial roles in initiating and responding to neuroinflammation.1 Astrocytes’ activation after injuries suggests that they could potentially serve as a marker…
Posted in NRG
