Summary We look at nanoparticle counting and colocalization after an ALine device membrane capture and compare IMARIS and ImageJ methods with an example. Procedures for particle counting and colocalization for IMARIS are discussed and a walkthrough is provided An example…
Category: NRG
The Enemy Within: Optimizing an OLCN PUA Coated Microporous Membrane Mimetic and Exposing an Unlikely Saboteur
The battle for soft membrane coatings wages on as we continue to optimize polyurethane acrylate (PUA) coatings for our osteomyelitis project, whose background is described here and here and initial progress is described here. Since our last update, we have…
Posted in NRG
Aliens, Voodoo, or Magic? How does DNA Extraction via Silica Adsorption Actually Work?
The first DNA extraction was done over 150 years ago in 18691. Since then, the process has been refined and expanded to include multiple physical and or chemical methods. For the waste water epidemiology project (described here), we will be…
Posted in NRG
Daily In-Situ Permeability using mouse CMECs
Introduction In the Glading Lab we study Cerebral Cavernous Malformations (CCMs). In CCMs, brain capillaries develop enlarged lumens and a disrupted blood-brain barrier. The presence of a mutation in KRIT1 causes loss of KRIT1 protein function in the endothelial cells.…
Flow/No Flow Conditions for Affinity Based Capture of EVs
In previous experiments where the affinity based capture of EVs in ALine devices was tested, issues were encountered. It was noticed that when capturing EVs on the ALine, the non-biotinylated slit membranes had captured a higher quantity of EVs compared…
Posted in NRG
Hemocompatibility Assessment of Microfluidic Extra Corporeal Membrane Oxygenator
Introduction Risks of extracorporeal membrane oxygenation (ECMO) therapy can be attributed to large membrane areas and blood circuit volumes. These are particularly problematic in neonates with low blood volume. To address this, we are developing a miniaturized prototype microfluidic ECMO…
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Little Back Dots in Coated Controls … How We Wonder What You Are.
Introduction We are investigating some issues seen with long term attachment (up to 6 days) of the EECM-BMEC-like cells seen by collaborators in Bern, where they noticed black aggregates on the coated control devices that get worse at day 6.…
Fabricating Thin Polymer Coatings to Study S. Aureus Durotaxis – PUA Optimization
Introduction The work in this post explores the hypothesis that durotaxis affects the invasion of S. aureus into the osteocyte lacuna-canicular network (OLCN). To address this hypothesis, we modified the µSiM to enable the independent tuning of pore stiffness, ranging…
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I’ve looked at cloudiness from both sides now…
“I’ve looked at clouds from both sides now” is a Joni Mitchell song. Some Background We are developing a white blood cell (WBC) detector to surveil peritoneal dialysate fluid during fluid exchanges for early detection of peritonitis. This preemptive monitoring…
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Who’s Your Cabby? Analyzing the Roles of Haptotaxis and Durotaxis on Staphylococcus Aureus Invasion of the Osteocyte Lacuna-Canicular Network
As discussed in a previous post of mine, I am currently working on a project to develop an in vitro mimetic of the osteocyte lacuna-canicular network (OLCN) to improve the ability to predict Staphylococcus Aureus invasion and colonization of the…
Posted in NRG
